摘要
目的研究膜联蛋白A1（annexin A1，ANXA1）在胰腺癌中的表达及其与增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）的柏关眭方法应用组织芯片制作仪构建芯片，芯片上有39例正常胰腺组织，42例胰腺癌组织，7例胰岛细胞僖组织，8例壶腹腺癌组织。在连续的芯片切片上使用免疫组化法检测ANXA1和PCNA蛋白的表达，分忻两者的相关性。结果构建的组织芯片在免疫组化染色后的掉片率为11.7％～13．3％，满足实验安求。ANXA1在胰腺癌中的阳性表达率为71．4％（30／42），明显高于正常胰腺组织18．4％（7／38）的阳性率（P〈0．01）。PCNA在胰腺癌中的15H性表达率为73．8％（31／42），也明显高于正常胰腺组织22．2％（8／36）的阳性率（P〈0．01）胰腺癌组织ANXA1表达与PCNA表达显著相关（P〈0．01）。结论胰腺癌ANXA1蛋白的表达与PCNA表达同步增加.
Objective To investigate the expressions of the annexin A1 （ANXA1） in pancreatic cancer and the correlations with proliferating cell nuclear antigen （PCNA）. Methods Tissue mieroarray instrument was used to construct the chip. There were 39 samples of normal pancreas tissue, 42 samples of pancreatic cancer, 7 samples of islet cell tumor and 8 samples of ampullary carcinoma. The expressions of ANXA1 and PCNA protein were examined by immunohistochemistry and their correlation was analyzed. Results The off-chip rate of tissue chips ＇after immunobistochemistry was 11.7% - 13.3% , which could satisfy the needs for experiment. Immuuohistochemical results of pancreatic cancer tissue microan＇ay showed that the positive rate of ANXA1 expression in pancreatic cancer was 71.4% （30/42）, which was significantly higher when compared with that of normal pancreas tissue （ 18.4% , 7/38 ; P 〈 0.01 ）. The positive rate of PCNA expression in pancreatic cancer was 73. 8% （ 31/42 ） , which was significantly higher than that of normal pancreas tissue （22.2% , 8/36; P 〈 0.01 ）. The expression of ANXA1 had a significant correlation with PCNA in pancreatic cancer （ P 〈 0.01 ）. Conclusions The expression of ANXA1 protein was significantly increased in pancreatic cancer. The expression of ANXAI,as significantly correlated with the expression of PCNA.
出处
《中华胰腺病杂志》
CAS
2010年第5期326-328,共3页
Chinese Journal of Pancreatology
基金
北京市自然科学基金资助项目（7082057）北京市科技新星计划（2007A105）
关键词
胰腺肿瘤
膜联蛋白A1
增殖细胞核抗原
组织芯片
Pancreatic neoplasms
Annoxin AI
Proliterating cell nuclear antigen
Tissue mieroarray
