摘要
目的:观察丹参酮Ⅱ_A磺酸钠注射液对大鼠急性心肌缺血/再灌注损伤缺血预处理后血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)表达的影响。方法:16只健康大鼠随机分为缺血/再灌注组、丹参酮预处理组,丹参酮预处理组经尾静脉注射1%丹参酮Ⅱ_A磺酸钠注射液,缺血/再灌注组给予等体积生理盐水预处理。于预处理14 d后采用结扎左侧冠状动脉前降支30 min再复灌30 min的方法建立大鼠急性心肌缺血/再灌注损伤模型。检测缺血预处理前后静脉血超氧化物歧化酶(Superoxide Dismutase,SOD)、过氧化氢酶(Catalase,CAT)活性及缺血心肌组织丙二醛(Malondialdehyde,MDA),心肌缺血区组织VEGF及VEGF-mRNA、缺氧诱导因子-1a(Hypoxia Inducible Factor-1a,HIF-1a)及HIF-1a mRNA、受体胎肝激酶-1(Fetal Liver Kinase 1,FLK-1)及FLK-1mRNA表达。结果:与缺血/再灌注组比较,丹参酮预处理组VEGF及VEGF-mRNA、FLK-1、FLK-1mRNA、HIF-1a、HIF-1amRNA的表达均明显增强,SOD及CAT活性明显提高,MDA降低(P<0.05)。结论:丹参酮Ⅱ_A磺酸钠预处理可提高VEGF表达及超氧化物歧化酶活性,对急性心肌缺血/再灌注损伤大鼠心肌有一定的保护作用。
Objective： To observe the effect of sodium sulfonate A sulfonate injection on the expression of vascular endothelial growth factor（VEGF） in rats with acute myocardial ischemia/reperfusion injury. Methods： Sixteen healthy rats were randomly divided into two groups,ischemia/reperfusion group and Salvia miltiorrhiza group. The two rats were treated with 1% sodium sulfonateⅡ A sulfonate injection and sodium chloride injection from caudal vein. After fourteen days,rats underwent a 30 min left anterior descending coronary artery occlusion and a 30 min reperfusion. This process was repeated 3 times. Mycardial tissue and serum were assessed by Western blot,quantitative RT-PCR and assay kit. Results： The expression of FLK-1,FLK-1mRNA,HIF-1a,HIF-1amRNA,VEGF and VEGF-mRNA were increased. The activity of SOD and CAT were significantly enhanced,however the content of MDA was decreased compared with IR. The difference showed statistically significant（P〈 0. 05）. Conclusion： Sodium Sulfonate A Sulfonate may enhance the expression of VEGF and the activity of SOD. It has certain protective effects on rats with acute myocardial ischemia/reperfusion injury.
出处
《世界中医药》
CAS
2016年第10期2105-2108,共4页
World Chinese Medicine
基金
十堰市科技局项目(编号:ZD2013013)
关键词
丹参酮ⅡA磺酸钠
急性心肌缺血预处理
血管内皮生长因子
Sodium Sulfonate A Sulfonate
Acute myocardial ischemia preconditioning
Vascular endothelial growth factor
