摘要
目的探讨1型糖尿病患者辅助性T细胞17(Th17细胞)比例及其相关细胞因子表达水平的变化。方法选取2012年1月—2014年10月在青海大学附属医院住院且符合纳入标准的1型糖尿病患者20例(1型糖尿病组)、2型糖尿病患者21例(2型糖尿病组),并选取同期本院体检健康者22例作为对照组。采用流式细胞仪检测外周血Th17细胞比例,采用ELISA法检测血浆白介素(IL)-17A、IL-23、IL-6、IL-1β水平。比较3组受试者一般资料〔体质指数(BMI)、年龄、收缩压(SBP)、舒张压(DBP)、球蛋白(GLB)、白细胞计数(WBC)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、尿素氮(BUN)、肌酐(Cr)、总胆固醇(TC)、三酰甘油(TG)、空腹血糖(FBG)〕,2型糖尿病组、1型糖尿病组患者临床指标〔尿酮、空腹C肽(FCP)、空腹胰岛素(FINS)、糖化血红蛋白(HbA_(1c))、胰岛素抗体(INS-Ab)、谷氨酸脱羧酶抗体(GAD-Ab)、病程〕,3组受试者Th17细胞比例、IL-17A水平、IL-23水平、IL-6水平、IL-1β水平;分析3组受试者Th17细胞比例和IL-17A水平、IL-23水平、IL-6水平、IL-1β水平的相关性,并采用多元线性回归分析分析Th17细胞比例、IL-17A水平、IL-23水平、IL-6水平、IL-1β水平与一般资料和临床指标的关系。结果 2型糖尿病组患者BMI、FBG水平高于对照组,SBP低于对照组(P<0.05);1型糖尿病组患者BMI、SBP、DBP、Cr水平、TC水平低于2型糖尿病组、对照组,FBG水平高于2型糖尿病组、对照组(P<0.05);1型糖尿病组患者TG水平低于2型糖尿病组(P<0.05)。1型糖尿病组患者HbA_(1c)、INS-Ab、GAD-Ab水平高于2型糖尿病组,尿酮、FCP、FINS水平低于2型糖尿病组(P<0.05)。3组受试者IL-6水平比较,差异无统计学意义(P>0.05);2型糖尿病组患者Th17细胞比例、IL-17A水平高于对照组(P<0.05);1型糖尿病组患者Th17细胞比例、IL-17A水平、IL-23水平、IL-1β水平高于2型糖尿病组、对照组(P<0.05)。1型糖尿病组
Objective To investigate the ratio of helper T cells 17（ Th17 cells） and the changes of the expression levels of its related cytokines in patients with type1 diabetes mellitus（ T1DM）. Methods According to the inclusion criteria,20 patients with T1DM（ T1DM group） and 21 patients with type2 diabetes mellitus（ T2DM group） hospitalized in Qinghai University Affiliated Hospital from January 2012 to October 2014 were selected, and 22 healthy people underwent physical examination in this hospital at the same period were enrolled as the control group. The proportion of Th17 cells in peripheral blood was detected by flow cytometry,the serum concentrations of interleukin 17A（ IL-17A）, IL-23, IL-6, IL-1β were measured by Enzyme- linked immunosorbentassay（ ELISA）. The general information 〔the body mass index（ BMI）, age,systolic blood pressure（ SBP）,diastolic blood pressure（ DBP）,globulin（ GLB）,white blood cell count（ WBC）,alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,blood urea nitrogen（ BUN）,creatinine（ Cr）,total cholesterol（ TC）,triacylglycerol（ TG）,fasting plasma glucose（ FBG） 〕of subjects in the three groups,clinical indicators of patients in T1DM group and T2DM group 〔the urine ketone body, fasting C- peptide（ FCP）, fasting insulin（ FINS）, glycosylated hemoglobin（ HbA1c）,insulin antibody（ INS- Ab）,glutamate acid decarboxylase antibody（ GAD- Ab）, course of disease〕,the ratio of Th17 cells and levels of IL-17 A,IL-23,IL-6 and IL- 1β of subjects in the three groups were compared. The correlation between the ratio of Th17 cells and the levels of IL-17 A,IL- 23,IL-6,IL-1β of subjects in the three groups were analyzed. Multiple linear regression analysis was used to analyze the relationship of Th17 cells ratio,levels of IL-17 A,IL-23,IL-6,and IL-1β with the general information and the clinical indicators. Results The BMI and FBG of patients in T2DM group were higher than those in control group,while SBP wa
作者
赵成玉
黄香丽
李占全
崔森
Mellitus ZHAO Cheng - yu HUANG Xiang - li LI Zhan - quan CUI Sen(Qinghai University Affiliated Hospital, Xining 810001, China)
出处
《中国全科医学》
CAS
北大核心
2017年第9期1061-1066,共6页
Chinese General Practice
基金
青海大学附属医院中青年科研基金项目(ASRF-2010-18)
