摘要
目的考察石斛属药用植物内生真菌拮抗软腐病的真菌。方法将179株内生真菌进行金钗石斛软腐病生物防治盆栽试验,以获得拮抗病原菌终极腐霉的内生真菌。将具有拮抗效果的真菌在PDA培养基中与终极腐霉进行双重培养平皿对峙试验并对其显微形态进行观察,利用3,5-二硝基水杨酸法检测真菌的β-1,3和β-1,4葡聚糖酶活性。结果 T.koningiopsis与T.rogersonii在盆栽试验和双重培养平皿对峙试验中对终极腐霉具有抑制作用。T.koningiopsis与T.rogersonii菌丝将终极腐霉菌丝包围并将其破坏;两者β-1,3和β-1,4葡聚糖酶活性在所测试的5种真菌中最高。结论 T.koningiopsis与T.rogersonii可有效抑制终极腐霉;由两者分泌的β-1,3和β-1,4葡聚糖酶可能在破坏终极腐霉细胞壁方面发挥作用。T.koningiopsis和T.rogersonii可作为潜在的生防菌发挥拮抗终极腐霉作用。
Objective To investigate the effects of the growth inhibition between P.ultimum and the potential biocontrol agents which have been isolated from Dendrobium species of medicinal plant.Methods Antagonistic effects of 179 endophytic fungi on P.ultimum in D.nobile Lindl.Seedlings in pot experiment were performed.In addition,the acitivities of β-1,3 glucanase and β-1,4 glucanase of 5 Trichoderma fungi in cell free filtrate were detected.The dual culture confrontation experiment between the antagonistic fungi and P.ultimum were also studied and the characteristics of the pathogen and the fungi with biocontrol activities were observed under light microscope.Results T.koningiopsis and T.rogersonii showed inhibitory effects on P.ultimum after cocultured with D.nobile Lindl.seedlings in pot experiment.The highest activity of β-1,3 glucanase and β-1,4 glucanase was produced by T.koningiopsis,which was followed by T.rogersonii.P.ultimum was damaged due to hyperparasitism by the two Trichoderma fungal strains.Conclusion After cocultured with D.nobile Lindl.,T.koningiopsis and T.rogersonii could effectively antagonize P.ultimum in pot experiment.The enzymes of β-1,3 glucanase and β-1,4 glucanase produced by Trichoderma species might be involved in destruction of P.ultimum cell wall during antagonism.T.rogersonii and T.koningiopsis could be used as potential biocontrol agents.
出处
《中国医药生物技术》
2017年第1期35-39,共5页
Chinese Medicinal Biotechnology
基金
山西省煤基重点科技攻关项目(FT2014-03)
河北省科技计划项目(16232503D)
