摘要
目的:通过对甘肃乌拉尔甘草野生与栽培品内生菌的分离,对甘草同属不同品种内生菌的优势菌种进行初步鉴定,比较二者的生理活性差异。方法:采用NA、PDA培养基对甘草野生与栽培品内生菌进行分离和纯化,并以分离频率比较判断各自优势菌种。结果:野生甘草分离到内生细菌65株,内生真菌7株,优势菌种为青霉菌属和曲霉属;栽培甘草分离到内生细菌54株,内生真菌6株,优势菌种为青霉菌属;野生与栽培甘草的内生真菌分属于6个属,相同的有5个属,不同的2个属为丝核菌属和木霉属。结论:野生与栽培甘草具有相趋同数量和种类的内生细菌与内生真菌,但二者内生真菌的菌属及优势菌种存在明显差异。
Objective： To compare the differences of biological activity between wild and cultivated glycyrrhiza uralensis through the isolation of endophytes and identification on dominant bacteria from endophytes of different species in the same genera.Method： Endophytes from wild and cultivated GanCao were isolated and purified with NA and PDA base.The dominant bacteria were compared and judged with frequency separation.Result： All 65 strains of endophytic bacteria and seven strains of endophytic fungi were isolated from wild GanCao,the dominant bacteria were penicillium spp and monillales;54 strains of endophytic bacteria and six strains of endophytic fungi were isolated from cultivated GanCao,the dominant bacteria were penicillium spp;endophytic fungi isolated from wild and cultivated GanCao were in the six genera,there were five same genera,two different genera were rhizoctonia and trichoderma.Conclusion： Wild and cultivated GanCao have almost the same amount and kinds of endophytic bacteria and fungi,but there exists remarkable difference in genera and dominant bacteria of endophtic fungi in both.
出处
《西部中医药》
2012年第11期8-11,共4页
Western Journal of Traditional Chinese Medicine
基金
甘肃省中医药管理局项目(编号GZK-2010-9)
关键词
甘肃
乌拉尔甘草
野生栽培
内生细菌
内生真菌
分离纯化
种类鉴定
Gansu
Glycyrrhiza Uralensis
wild cultivation
endophytic bacteria
endophytic fungi
isolation and purification
species identification
