摘要
目的探讨胃腺癌组织分化抗原151(CD151)、小凹蛋白-1(Cav-1)与膜型基质金属蛋白酶-1(MT1-MMP)的表达变化及其临床意义。方法选取2014年5月至2015年5月我院消化外科胃腺癌手术患者65例,术中采集胃腺癌组织标本65例作为研究组,癌旁正常胃黏膜组织标本65例作为对照组,采用SP免疫组化方法检测CD151、Cav-1与MT1-MMP蛋白表达水平。结果研究组CD151与MT1-MMP蛋白阳性表达率分别为78.46%和80.00%,明显高于对照组的6.15%和12.31%,Cav-1蛋白阳性表达率为52.31%,明显低于对照组的80.00%,两组比较差异均具有统计学意义(P<0.05);CD151、Cav-1与MT1-MMP蛋白表达与组织学分化程度、临床分期和淋巴结转移等临床病理特征具有明显的相关性,其中低分化及其未分化胃腺癌、中晚期胃腺癌及其有淋巴结转移胃腺癌CD151与MT1-MMP蛋白阳性表达率显著增高,Cav-1蛋白阳性表达率显著降低,不同临床病理特征胃腺癌组织CD151、Cav-1与MT1-MMP蛋白阳性表达率比较差异均具有统计学意义(P<0.05);经Pearson相关性分析显示,CD151、MT1-MMP与Cav-1表达之间具有明显的负相关(r=-0.521,-0.501,P<0.05),CD151与MT1-MMP表达之间具有明显的正相关(r=0.547,P<0.05)。结论胃腺癌组织CD151与MT1-MMP表达明显上调,Cav-1表达明显下调,三者均与临床病理特征具有紧密的关系。
Objective To investigate the expression of CD151,caveolin-1（Cav-1） and membrane type matrix metalloproteinase-1（MT1-MMP） and its clinical significance.Methods We recruited 65 patients of gastric cancer from May 2014 to May 2015 in our hospital.Gastric cancer specimens（n=65,the study group） were collected intraoperatively as study group,and the adjacent normal gastric mucosa tissues（n=65,the control group） were collected as control group,Expression of CD151,Cav-1 and MT1-MMP protein were detected by immunohistochemical method.Results The positive expression rates of CD151 and MT1-MMP in the study group were significantly higher than those in the control group（78.46% vs 6.15%,80.00% vs 12.31%）,while the positive expression rate of Cav-1 protein was significantly lower than that in the control group（52.31% vs 80.00%）,all with statistically significant differences（P〈0.05）.CD151,Cav-1and MT1-MMP protein expression had significant relevance with clinicopathological features of histological differentiation,clinical staging and lymph node metastasis.In patients with poorly and undifferentiated gastric adenocarcinoma,advanced gastric adenocarcinoma,and gastric adenocarcinoma complicating lymph node metastasis,the positive expression rate of CD151 and MT1-MMP protein was significantly increased,and the Cav-1 protein positive expression rate was significantly decreased.The positive expression rates of CD151,Cav-1 and MT1-MMP protein showed statistically significant difference among patient groups with different clinicopathological characteristics（P〈0.05）.Pearson correlation analysis showed that,there were significant negative correlations between CD151,MT1-MMP and Cav-1（r=-0.521,-0.501,P〈0.05）,and a significant positive correlation between CD151 and MT1-MMP（r=0.547,P〈0.05）.Conclusion In gastric cancer tissues,CD151 and MT1-MMP expression are up-regulated and Cav-1 expression is significantly down-regulated,which has close relationship with clinicopathological characteristics.
出处
《海南医学》
CAS
2017年第3期384-387,共4页
Hainan Medical Journal
基金
江苏省南京市医学科技发展项目(编号:QYX11172)
