摘要
目的探讨子宫颈鳞状细胞癌(cervix squamous cell carcinoma,CSCC)中整合素α5β1蛋白和mRNA的表达,并分析其与临床病理特征的关系。方法采用免疫组化SP法、RT-PCR技术检测60例CSCC及40例子宫颈正常组织中整合素α5β1蛋白及mRNA的表达。结果 CSCC中整合素α5β1蛋白及mRNA表达均高于子宫颈正常组织,差异有统计学意义(P<0.05);整合素α5β1蛋白及mRNA的表达均与CSCC分化程度、pTNM分期、淋巴结转移、浸润深度有相关性(P<0.05),与患者年龄无相关性(P>0.05)。结论 CSCC中整合素α5β1蛋白及mRNA的表达与其生长、侵袭与转移密切相关。采用免疫组化SP法与RT-PCR技术联合检测CSCC中整合素α5β1蛋白及mRNA,有望成为评估CSCC恶性程度、侵袭、转移和临床预后的指标。
Purpose To detect the expression of integrin α5β1 protein and mRNA in cervical squamous cell carcinoma （CSCC） and its relationship with pathological features. Methods By immunohistochemistry （ SP method） and reverse transcription-polymerase chain reaction （RT-PCR） the expression of integrin α5β1 protein and mRNA was detected in 60 cases of CSCC and 40 cases of normal tis- sues. Results Integrin α5β1 protein and mRNA in cervical CSCC were higher than that of normal cervical tissue, with statistically sig- nificant （P 〈0. 05）. In CSCC, the expression of integrin α5β1 mRNA and protein was correlated with the degree of differentiation, hormone level, pTNM stage, lymph node metastasis, and depth of invasion （ P 〈 0. 05 ）, but not with age （ P 〉 0. 05 ）. Conclusion Integrin α5β1 protein and mRNA expression is closely related to the growth, invasion and metastasis in CSCC. Application of immuno- histochemistry and RT-PCR technique to detect integrin α5β1 protein and mRNA can be used as indicators of the degree of malignancy, invasion and metastasis and prognosis evaluation in cervical squamous cell carcinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2014年第10期1127-1130,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
河北省人口计生委科研项目(2012-A21)
