摘要
目的:研究黏附分子整合素β1、粘着斑激酶(FAK)在喉鳞状细胞癌(LSCC)中的表达及临床意义。方法:应用RT-PCR和免疫组织化学SABC法,检测48例LSCC标本癌组织及其癌旁组织和20例声带息肉组织中整合素β1、FAK的表达情况。结果:LSCC中整合素β1和FAK蛋白及mRNA表达水平均高于癌旁组织和声带息肉组织,差异有统计学意义(P<0.05);LSCC中整合素β1和FAK蛋白及mRNA在有颈淋巴结转移组织中的表达明显高于无颈淋巴结转移组织,差异有统计学意义(P<0.05);在T3～T4期组织中的表达明显高于T1～T2期,差异有统计学意义(P<0.05);但与病理分级相比较其差异均无统计学意义(P>0.05)。结论:整合素β1和FAK在T3～T4期和有淋巴结转移的癌组织中表达明显升高,可能与喉癌的浸润、转移密切相关。
Objective：To study the expressions and clinical significance of integrin β1 and focal adhesion kinase （FAK） in laryngeal carcinoma （LSCC）. Method： The mRNA and protein levels of integrin β1 and focal adhesion kinase and the surrounding tissue of laryngeal carcinoma in 48 specimens and 20 specimens of vocal cord polyp were detected by reverse transcription-polymerase chain reaction （RT PCR） and immunohistochemistry SABC method. Result：The mRNA levels and positive rates of integrin β1 and FAK protein were significantly higher in laryngeal carcinoma than that in the surrounding tissue and vocal cord polyp （P〈0. 05）. The expression levels of integrin β1 and FAK were significantly higher in the group with cervical lymph node metastasis than those without （P〈0.05） ,and they were significantly higher in the tissue of stage of T3 and T4 than those of T1 and T2 （P〈0.05）. But pathological grades was not significantly related with the expression levels of integrin β1 or FAK （P〉0. 05）. Conclusion：The expression levels of integrin β1 and FAK were increased in LSCC, and they may contribute significantly to invasion and metastasis of LSCC.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2008年第24期1112-1114,共3页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
喉肿瘤
粘着斑激酶
整合素
逆转录聚合酶链反应
免疫组织化学
Laryngeal neoplasms
Focal adhesion kinase
Integrin
Reverse transcription polymerase chain reaction
Immunohistochemistry
