摘要
目的:建立高效液相色谱法同时测定甜叶菊叶中新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A和异绿原酸C6个酚酸类成分。方法:采用C18色谱柱(4.6 mm×250 mm,5μm),流动相为0.1%甲酸水溶液-乙腈,梯度洗脱,柱温30℃,检测波长327 nm。结果:6个酚酸质量浓度在10~196μg·m L-1范围内线性关系良好;6个酚酸的加样回收率分别为98.3%、95.2%、100.1%、97.3%、95.6%和96.1%,加样回收率、精密度、重复性、稳定性均符合有关规定;9批样品中6个酚酸类成分的含量分别为新绿原酸0.062%~0.725%,绿原酸0.277%~2.450%,隐绿原酸0.096%~0.694%,异绿原酸B 0.046%~0.867%,异绿原酸A0.157%~0.772%,异绿原酸C 0.135%~1.821%,6个酚酸之和在1.182%~6.668%。结论:该方法可为甜叶菊进一步开发利用提供质量控制方法。
Objective：To establish an HPLC method for simultaneous determination of six phenolic acids including 5-caffeoylquinic acid,chlorogenic acid,4-caffeoylquinic acid,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in the leaves of Stevia rebaudiana Bertoni.Methods：The analyses were performed on a C18 column（4-.6 mm×250 mm,5μm） with the gradient elution of acetonitrile-0.1% formic acid water at a flow rate of 1.0 mL ·min1.The detection wavelength was set at 327 nm and the column temperature was set at 30 ℃.Results：The six phenolic acids have good linearity in the concentration range of 10-196 μg·m L-1.The average recoveries of the six compounds were 98.3%,95.2%,100.1%,97.3%,95.6% and 96.1%,respectively.The contents of six phenolic acids in 9 samples were 0.062%-0.725%（5-caffeoylquinic acid）,0.277%-2.450%（chlorogenic acid）,0.096%-0.694%（4-caffeoylquinic acid）,0.046%-0.867%（3,4-dicaffeoylquinic acid）,0.157%-0.772%（3,5-dicaffeoylquinic acid） and 0.135%-1.821%（4,5-dicaffeoylquinic acid）,respectively.The sum of the sixphenolic acids is 1.182%-6.668%.Conclusion：The HPLC method is helpful for the quality control of the leaves of Stevia rebaudiana Bertoni.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2017年第2期219-223,共5页
Chinese Journal of Pharmaceutical Analysis
关键词
甜叶菊
甜菊
糖草
新绿原酸
绿原酸
隐绿原酸
异绿原酸
咖啡酰奎宁酸
酚酸
高相液相色谱
Stevia rebaudiana Bertoni
Tianju
Tangcao
neochlorogenic acid
chlorogenic acid
cryptochlorogenic acid
isochlorogenic acid
caffeoylquinic acid
phenolic acid
HPLC
