摘要
目的建立高效液相色谱法同时测定山银花提取物中7种有机酸的含量。方法准确称取一定量过筛的山银花提取物粉末经50%甲醇溶解并超声提取后,采用Acclaim 120 C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%磷酸溶液进行梯度洗脱,流速为1.0 m L/min,柱温为25℃,检测波长为327 nm,测定7种有机酸的含量。结果 7种有机酸在相应浓度区间线性良好(r>0.999),回收率为86.3%~97.7%,相对标准偏差<5%(n=6)。结论该方法操作简便、结果准确、灵敏度高,适用于山银花提取物中7种有机酸含量的测定。
Objective To establish a method for the determination of 7 kinds of organic acids in Lonicerae Flos extract by high performance liquid chromatography. Methods A certain amount of samples were weighed accurately, and dissolved with 50% methanol and extracted with ultrasounds. The samples were separated with a Acclaim 120 C18 column（4.6 mm×250 mm, 5 μm） at the flow rate of 1.0 m L/min by gradient elution using acetonitril and 0.4% phosphoric acid solution as the mobile phase. The temperature of the column oven was maintained at 25 ℃, the detection wavelength was 327 nm, and then the content of 7 kinds of organic acids was detected. Results The 7 kinds of organic acids showed a good linear correlation in certain concentration range（r0.999）, respectively. The average recoveries of 7 kinds of organic acids were 86.3%-97.7%, and the relative standard deviations were less than 5%（n=6）. Conclusion This method is simple, accurate and highly sensitive, which is suitable for the determination of 7 kinds of organic acids in Lonicerae Flos extract.
作者
涂晓琴
杨柳
罗艳
TU Xiao-Qin;YANG Liu;LUO Yan(Hunan Institute for Drug Control, Changsha 410001, China)
出处
《食品安全质量检测学报》
CAS
2018年第10期2457-2462,共6页
Journal of Food Safety and Quality
基金
湖南省自然科学基金项目(2017JJ3183)~~
关键词
山银花提取物
有机酸
高效液相色谱法
Lonicerae Flos extract
organic acids
high performance liquid chromatography
