摘要
采用香烟烟雾提取物刺激KB细胞,构建急性口腔炎症模型;用Elisa法测定炎症因子TNF-α、IL-6、IL-8和IL-10的表达量;以考察金银花、山银花抗炎活性的差异。结果显示,与空白组相比,模型组促炎因子TNF-α、IL-6、IL-8水平显著升高,抗炎因子IL-10水平显著降低,属急性炎症表现,说明造模成功。金银花提取物或山银花提取物处理后,均可抑制TNF-α、IL-6、IL-8表达水平的升高,改善IL-10分泌减少,且呈剂量依赖关系,提示金银花和山银花对口腔炎症均具有一定的治疗作用。对二者的药效结果比较,山银花对各炎症因子的调控作用均强于金银花,尤其对TNF-α、IL-6和IL-10的调控作用具有显著差异。本研究从药效角度对金银花和山银花进行比较研究,为合理利用金银花和山银花药材提供了依据。
The acute stomatitis model in KB cells induced by cigarette smoke extract（ CSE） has been established to study the anti-inflammatory effects between Lonicerae Japonicae Flos（ LJF） and Lonicerae Flos（ LF）,and the protein expression levels of TNF-α,IL- 6,IL- 8 and IL- 10 were detected by Elisa method after treatments of LJF or LF extracts. The results showed that the extracts of LJF or LF could both reduce the protein expression levels of TNF-α,IL- 6,IL- 8,improve the low expression of IL- 10 in a dose dependent manner,suggesting their certain anti-inflammatory effects. LF presented significant better anti-inflammatory effects than LJF in the regulation of TNF-α,IL- 6 and IL- 10. These results provided a basis for the rational utilization of LJF and LF herbs.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第4期118-122,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家科技支撑计划资助项目(2012BAI29B00)
广州开发区科技领军人才资助项目(2015P-158)
关键词
金银花
山银花
KB细胞
急性口腔炎症
Lonicerae Japonicae Flos
Lonicerae Flos
KB cells
acute stomatitis model
