摘要
目的:利用HPLC-Q-TOF/MS分析技术快速、系统地鉴定白屈菜中异喹啉类生物碱。方法:采用XAqua C8色谱柱(150 mm×2.1 mm,5μm);以乙腈-0.1%甲酸水溶液为流动相梯度洗脱;体积流量为0.3 m L·min-1;ESI源正离子模式检测。结合对照品的二级质谱及相关文献推测生物碱的结构。结果:从白屈菜的甲醇提取物中鉴定了18个生物碱,其中木兰箭毒碱(1)、北美黄连碱(4)、药根碱(12)、非洲防己碱(14)为首次在白屈菜中报道。结论:HPLC-Q-TOF/MS方法能快捷、准确地鉴定白屈菜中的化学成分,为白屈菜化学成分的进一步提取分离和药效物质基础的研究提供科学依据。
Objective：To systematically and rapidly identify isoquinoline alkaloids in Chelidonium majus using the HPLC-Q-TOF/MS.Methods：The separation was performed on a XAqua C8column（150 mm×2.1 mm,5 μm）with acetonitrile-0.1% formic acid in water by gradient elution.The flow rate was 0.3 mL·min^-1.Electrospray ionization-（ESI）source was applied and operated in positive ion mode.The structure of alkaloids was speculated by MS/MS fragmentation behaviors of standards and related references.Results：18 alkaloids were elucidated from the methanolic extracts of Ch.majus.Compounds magnocurarine（1）,canadine（4）,jatrorrhizine（12）,columbamine（14）were reported firstly in Ch.majus.Conclusion：HPLC-Q-TOF/MS is rapid and reliable method for identification of the alkaloids in Ch.majus.The results would provide scientific evidence for further extraction and separation of components and study of pharmacodynamic material basis in Ch.majus
出处
《中国现代中药》
CAS
2017年第2期174-182,共9页
Modern Chinese Medicine
基金
长沙市科技计划重点项目(K1308182-21)
