摘要
目的:优选白屈菜粗多糖的水提醇沉工艺。方法:以白屈菜多糖得率和多糖含量为指标,以水为溶剂,选取料液比、提取时间、提取温度、提取次数、醇沉浓度为影响因素,进行单因素考察,在综合单因素实验的基础上,采用L9(3~4)正交试验,以加权评分法综合评估白屈菜多糖提取条件,对白屈菜多糖水提工艺进行优化,采用苯酚-硫酸法测定多糖的含量。结果:白屈菜多糖的最佳提取工艺为30倍量的水,100℃提取3次,每次3h,滤液浓缩至料液比1∶4,加入95%乙醇至含醇量达到80%,静置过夜,离心,沉淀,依次用无水乙醇、丙酮、无水乙醚洗涤,挥干乙醚即得。结论:此工艺操作简便、成本低廉、重现性好、适合大规模工业生产,可用于白屈菜药材及饮片中多糖的提取。
Objective： To optimize water extraction and alcohol precipitation process of polysaccharide from Chelidonium majus. Methods： On the basis of single factor test with materia liquid ratio, extraction time, extract temperature, extraction times and alcohol concentration of alcohol-precipitation procedure for influence factors. On the basis of single factor experiment,the orthogonal test with 4 factors and 3 levels method was used to design and the content of the extract was determined using anthrone-sulfuric acid colorimetry method. Results： The best extracting process was carried out by water（1 ： 30）, extracting 3 times under 100℃, each time 3 hours. The filtrate is concentrated to the mixture ratio of 1 ：4 and the 95% ethanol was added to the alcohol content reaches 80%, rest over night, centrifuge, the precipitation in turn is washed with anhydrous ethanol, acetone, anhydrous ether, with the drying of ether. Conclusion：This process is simple, inexpensive, reproducible and suitable for large-scale industrial production, It can be used for the extraction of polysaccharides from Chelidonium majus.
作者
刘蕾
冯素香
刘富岗
张杰
Liu Lei;Feng Suxiang;Liu Fugang;Zhang Jie(Pharmacy Department,Children＇s Hospital Affiliated to Zhengzhou University,Zhengzhou 450053,China;Pharmacy Department,Henan Children＇s Hospital,Zhengzhou 450053,China;Pharmacy Department,Zhengzhou Children＇s Hospital,Zhengzhou 450053,China;Pharmacy College,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Respiratory Diseases Diagnosis and＆Chinese Medicine Development of Henan province,zhengzhou 450046,China;Henan Key Laboratory of Chinese Medicine for Respiratory Disease,Zhengzhou 450046,China)
出处
《亚太传统医药》
2018年第10期31-35,共5页
Asia-Pacific Traditional Medicine
基金
国家自然科学基金青年基金(81603556)
关键词
白屈菜
多糖
正交试验
苯酚-硫酸法
Chelidonium majus L.
Polysaccharide
Orthogonal Test
Phenol-sulfuric Acid Method
