摘要
目的考察入山虎Zanthoxylum nitidum var.tomentosum不同提取部位对类风湿性关节炎(rheumatoid arthritis,RA)的治疗作用及提取部位的化学成分分析。方法考察入山虎不同提取部位对人RA成纤维样滑膜MH7A细胞增殖活性和炎症因子含量的影响,通过对大鼠足趾部皮内注射完全弗氏佐剂建立RA大鼠模型,测定大鼠体质量、全身评分、关节炎指数、足跖肿胀度、脏器系数、血清中炎症因子含量,采用苏木素-伊红染色观察踝关节组织病理,以评价入山虎不同提取部位的抗RA活性作用。采用UPLC-Q-TOF-MS/MS分析入山虎提取部位的化学成分。结果入山虎中的氯仿及石油醚、醋酸乙酯、正丁醇、水提取部位在体内外对RA均具有良好的抗炎作用。在体外,能够抑制MH7A细胞的增殖(P<0.05、0.01),减少炎症因子白细胞介素-6(interleukin-6,IL-6)、IL-17A、IL-1β的分泌(P<0.01);在体内,入山虎的提取部位可不同程度降低RA大鼠的足跖肿胀度、全身评分及关节炎评分(P<0.05、0.01),改善踝关节的病理改变,缓解RA大鼠的炎症反应,还能减少促炎因子IL-6、IL-17A、IL-1β的分泌(P<0.01),增加抗炎因子IL-4、IL-10的分泌(P<0.01)。对入山虎的提取部位进行化学成分分析,发现入山虎的提取物含有的化合物比较相似,含有生物碱、黄酮、香豆素等其他化合物,以生物碱类居多,主要包括呋喃喹啉类、苄基异喹啉类、原小檗碱类、阿朴啡类、苯并菲啶类、酰胺类等。结论入山虎中的氯仿及石油醚、醋酸乙酯、正丁醇、水部位在体内外对RA均具有良好的抗炎作用,入山虎提取物主要含有生物碱、黄酮、香豆素等其他化合物。
Objective To investigate the effect of different extraction parts from Zanthoxylum nitidum var.tomentosum(ZN)on rheumatoid arthritis(RA)and chemical composition analysis of extraction parts.Methods The human RA fibroblast-like synoviocyte cell line MH7A cells were used to investigate the effects of different extraction parts of ZN on the proliferation activity and contents of inflammatory factors.RA rat model was established by intradermal injection of Freund’s complete adjuvant into the toes of rats.The body weight,whole body score,arthritis score,degree of swelling,organ coefficient and contents of inflammatory factor of rats were measured,pathology of ankle joint tissue was used by HE staining to evaluate the anti-RA activity of different extraction parts of ZN.And the chemical constituents of the extract were analyzed by UPLC-Q-TOF-MS/MS.Results Chloroform,petroleum ether,ethyl acetate,n-butanol and water extraction parts of ZN had good anti-inflammatory effects on RA in vivo and in vitro.ZN could inhibit the proliferation of MH7A cells in vitro(P<0.05,0.01),reduce the secretion of inflammatory factors interleukin-6(IL-6),IL-17A and IL-1β(P<0.01).In vivo,the extraction parts of ZN could reduce the paw swelling,whole body score and arthritis score of RA rats to varying degrees(P<0.05,0.01),improve the pathological changes of ankle joint,alleviate the inflammatory symptoms of RA rats,reduce the secretion of pro-inflammatory factors IL-6,IL-17A and IL-1β(P<0.01),increase the secretion of anti-inflammatory factors IL-4 and IL-10(P<0.01).The chemical constituent analysis showed that the extracts of ZN contained similar compounds,including alkaloids,flavonoids,coumarins and other compounds.Most of them were alkaloids,including furan quinolines,benzylisoquinolines,protoberberines,aporphines,benzophenanthridines,amides and so on.Conclusion Chloroform,petroleum ether,ethyl acetate,n-butanol and water extraction parts of ZN have good anti-inflammatory effects on RA in vivo and in vitro.The extract mainly contains a
作者
曾英港
王柳萍
奉建芳
唐丹丹
蒙泔竹
ZENG Ying-gang;WANG Liu-ping;FENG Jian-fang;TANG Dan-dan;MENG Gan-zhu(Guangxi University of Chinese Medicine,Nanning 530200,China;Jiangxi University of Chinese Medicine,Nanchang 330004,China;Guangxi Superior Chinese Patent Medicine and National Medicine Development Engineering Technology Research Center,Nanning 530020,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第13期4186-4201,共16页
Chinese Traditional and Herbal Drugs
基金
国家重点研发计划项目(2019YFC1712304)
广西科技基地和人才专项(桂科AD20238058)
广西壮瑶药重点实验室项目(GXZYKF2022-16)
广西中医药管理局科研课题(GXZYZ20210068)。
关键词
入山虎
不同提取部位
类风湿性关节炎
炎症因子
生物碱
黄酮
香豆素
木兰花碱
橙皮苷
两面针碱
Zanthoxylum nitidum(Roxb.)DC.var.tomentosum Huang
different extraction parts
rheumatoid arthritis
inflammatory factors
alkaloids
flavonoids
coumarins
magnoflorine
hesperidin
nitidine
