摘要
目的探讨三七皂苷、三七黄酮、三七氨酸及其混合物对全周期持续外源性雌激素干预下的未成年大鼠子宫内膜组织因子(TF)、组织因子途径抑制物2(TFPI-2)表达的影响。方法将160只雌性未成年大鼠随机分成空白组(40只)和模型组(120只),空白组给予生理盐水灌胃3周,模型组给予戊酸雌二醇悬液灌胃3周。在全周期(3周)干预后,将无性周期大鼠随机分成6组,即模型组、连续雌激素组、三七皂苷组、三七黄酮组、三七氨酸组、三七有效部位混合组。并分别给予生理盐水、雌激素、三七皂苷、三七黄酮、三七氨酸、三七有效部位混合物,连续灌胃2周。2周后检测子宫内膜中TF、TFPI-2及TF、TFPI-2 mRNA表达的水平。结果与空白组比较,模型组TF、TF mRNA阳性表达增高,TFPI-2、TFPI-2 mRNA阳性表达降低,差异有统计学意义(P<0.05,P<0.01);第5周末,与模型组比较,雌激素组的TF、TF mRNA表达增高,TFPI-2、TFPI-2 mRNA表达降低,差异有统计学意义(P<0.01);与模型组和雌激素组比较,三七皂苷组、三七黄酮组、三七氨酸组、三七混合组TF、TF mRNA阳性表达均降低,TFPI-2、TFPI-2 mRNA阳性表达均增高,差异有统计学意义(P<0.05,P<0.01);与三七皂苷组比较,三七混合组TFPI-2、TFPI-2 mRNA阳性表达增高,差异有统计学意义(P<0.05);与三七黄酮组比较,三七皂苷组、三七混合组TF、TF mRNA表达降低;三七混合组TFPI-2阳性表达增高,差异亦有统计学意义(P<0.05,P<0.01)。结论三七有效部位可降低全周期持续外源性雌激素干预大鼠子宫内膜TF表达,升高TFPI-2表达,其可能机制是通过抑制TF,阻断凝血系统激活,减少炎症反应,而达到活血止血的目的;同时,可能加强子宫内膜细胞外基质,维持子宫内膜稳定性,而减少内膜崩解出血,有利于内膜修复、重塑作用。
Objective To observe the effects of notoginsenoside,Panax notoginseng flavonoids（ PNF）,Panax notoginseng acid（ PNA）,and their mixtures on the expressions of tissue factor（ TF） and tissue factor pathway inhibitor 2（ TFPI-2） in rat endometriaum with whole cycle exogenous estrogen intervention. Methods Totally 1 6 0 female impuberty rats were randomly divided into the blank group（n =40） and the model group（n = 120）. Rats in the blank group were administered with normal saline by gastrogavage for 3 weeks,while those in the model group were administered with estradiol valerate suspension.The two interventions lasted for 3 consecutive weeks. After 3 weeks of intervention,rats with asexual cyclewere randomly divided into six groups,i. e.,the model group,the continuous estrogen group,the Notoginsenoside group,the PNF group,the PNA group,the mixture group. Rats in the model group,the continuous estrogen group,the Notoginsenoside group,the PNF group,the PNA group,the mixture group were respectively administered with normal saline,estrogen,notoginsenoside,PNF,PNA,and mixture of effective Panax notoginseng fractions by gastrogavage for 2 successive weeks. Expression levels of TF,TFPI-2,TF mRNA,and TFPI-2 mRNA in the endometrium were detected 2 weeks later. Results Compared with the blank group,positive expressions of TF and TF mRNA increased,and positive expressions of TFPI-2 and TFPI-2 mRNA decreased in the model group（P〈0.05,P〈0.01）. Compared with the model group,the expressions of TF and TF mRNA significantly increased,the expressions of TFPI-2 and TFPI-2mRNA significantly decreased in the estrogen group at the end of the 5 th week（P〈0.01）. Compared with the model group and the estrogen group,positive expressions of TF and TF mRNA significantly decreased,positive expressions of TFPI-2 and TFPI-2 mRNA significantly increased in the Notoginsenoside group,the PNF group,the PNA group,the mixture group（P〈0.05,P〈0.01）. Compared with the Notoginsenoside group,positive expressions of
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2017年第2期215-219,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金项目(No.30271633)
陕西中医药大学自然科学培育基金(No.2015PY15)
关键词
三七皂苷
三七黄酮
三七氨酸
组织因子
组织因子途径抑制物2
notoginsenoside
Panax notoginseng flavonoids
Panax notoginseng acid
tissue factor
tissue factor pathway inhibitor 2
