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TFPI-2基因对Panc-1细胞凋亡的影响

The Effect of TFPI-2 on Apoptosis of Pancreatic Carcinoma Cell Panc-1
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摘要 [目的]研究TFPI-2基因对胰腺癌细胞系Panc-1细胞增殖及凋亡的影响。[方法]测定Panc-1-TFPI-2、Panc-1-V和Panc-1-P三组细胞的生长曲线,DNA片段化实验检测细胞凋亡,并用流式细胞仪分析三组细胞的细胞增殖、细胞周期情况。[结果]Panc-1-TFPI-2细胞同Panc-1-V和Panc-1-P细胞相比,细胞生长缓慢,细胞生长受到抑制,被阻滞于G0~G1期;DNA片段化实验显示Panc-1-TFPI-2细胞呈现凋亡细胞特有的"梯状"条带,而Panc-1-V和Panc-1-P细胞无明显"梯状"条带;流式细胞仪分析显示早期Panc-1-TFPI-2细胞凋亡率(6.93%±0.53%)较Panc-1-V(3.01%±0.39%)和Panc-1-P细胞凋亡率(3.52%±0.41%)增加,有显著性差异(P<0.05)。[结论]TFPI-2能够抑制胰腺癌细胞生长、诱导胰腺癌细胞凋亡,可能具有抑制肿瘤细胞增殖的作用。 [Purpose] To investigate the effect of TFPI-2 gene on cell proliferation and apoptosis of human pancreatic cancer cell line Pane-1. [Methods] The growth curve was detected in 3 groups, Panc-1-TFPI-2, Panc-1-V and Panc-1-P.DNA strand breaks was used to detect the apoptosis in the 3 groups. The change of cell cycle and apoptosis index were evaluated by flow cytometry. [Resuhs] Compared with nontransfected cells (Pane-1-P, Pane-1-V), the growth of Pane-1-TFPI-2 cell was slower and inhibited significantly.The transfeeted cells showed a significant increase in G0-G1 phase.The apoptosis of Panc-1-TFPI-2 cell was identified by DNA strand breaks and flow cytometry in comparison with the control group.The apoptosis index of the Panc-1-TFPI-2 cell (6.93%± 0.53%) was higher than that in Panc-1-V (3.01%± 0.39%) and Pane-1-P (3.52%± 0.41%), with significant difference(P〈0.05). [Conclusion] The exogenous TFPI-2 gene can inhibit the growth and induce apoptosis of pancreatic cancer cell, which maybe inhibit the cancer cells proliferation.
出处 《肿瘤学杂志》 CAS 2008年第3期183-185,共3页 Journal of Chinese Oncology
基金 安徽省自然科学基金资助项目(050430702)
关键词 胰腺肿瘤 细胞凋亡 组织因子途径抑制物2(TFPI-2) pancreatic neoplasms apoptosis tissue factor pathway inhibitor 2
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参考文献8

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