摘要
目的建立复方磺胺甲噁唑片中磺胺甲噁唑和甲氧苄啶含量测定的双波长分光光度法。方法采用双波长分光光度法测定。磺胺甲噁唑的测定波长为257 nm,参比波长为305 nm,以0.4%氢氧化钠溶液作为SMZ的空白溶剂;甲氧苄啶的测定波长为239 nm,参比波长为293 nm,以盐酸—氯化钾溶液作为TMP的空白溶剂。结果磺胺甲噁唑浓度在2~10μg·ml^(-1)之间,甲氧苄啶浓度在0.2~1.0μg·ml^(-1)之间,符合比尔定律,浓度与吸收度差值呈良好的线性关系。两被测成分的平均回收率和RSD分别为磺胺甲噁唑97.84%和0.25%,甲氧苄啶为:97.91%和0.2%。结论该方法简便、准确,可作为该片剂质量控制的方法之一。
Objective： A dual wavelength determination of sulfamethoxazole and trimethoprim in compound sulfamethoxazole tablets by spectrophotometry. Methods： Determined by dual wavelength spectrophotometry. Wavelength determination of sulfamethoxazole was 257 nm,the reference wavelength was 305 nm. Taking 0. 4% sodium hydroxide solution as SMZ blank solvent. Wavelength determination of trimethoprim was 239 nm,the reference wavelength was 293 nm. Hydrochloric acid potassium chloride solution as the TMP blank solvent. Results： The results showed that both the standard curve of sulfamethoxazole concentration ranging from 2 to 10 μg·ml- 1and trimethoprim concentration ranging from 0. 2 to 1. 0 μg·ml- 1was in correspondence with Beer＇s law. The absorptivity difference and the concentration showed a good linear relationship. The average recoveries and RSD were 97. 84% 、0. 25% for sul-famethoxazole,and were 97. 91% 、0. 2% for trimethoprim. Conclusion： The method is simple and accurate,which can be used as one of methods for quanlity control of the tables.
作者
王乐婷
郭秀琴
李珂
WANG Le-ting GUO Xiu-qin LI Ke(Taishan Medical College, Taian 271016, China)
出处
《泰山医学院学报》
CAS
2016年第12期1336-1339,共4页
Journal of Taishan Medical College
关键词
双波长分光光度法
磺胺甲噁唑
复方磺胺甲噁唑片
甲氧苄啶
dual wavelength spectrophotometry
sulfamethoxazole tables
sulfamethoxazole compound
trimethoprim
