摘要
[目的]建立一种利用高效液相色谱测定80%吡蚜酮·烯啶虫胺水分散粒剂的定量分析方法。[方法]采用ODS-2反相色谱柱,紫外检测器,以乙腈/水/磷酸盐缓冲液(体积比10∶80∶10)为流动相,对试样中吡蚜酮和烯啶虫胺进行分离,外标法定量测定。[结果]吡蚜酮和烯啶虫胺线性相关系数分别为0.9995和0.9998,方法标准偏差为0.29和0.22。变异系数分别为0.51%和1.21%,平均回收率分别为100.7%和99.1%。[结论]该方法简便、快捷,有效地分离了样品中的吡蚜酮和烯啶虫胺,而且精密度和准确度高、线性关系好。
[Aims] A HPLC method for quantitive determination ofpymetrozine-nitenpyram 80% WG was established. [ Methods ] The pymetrozine and nitenpyram in the samples were separated by ODS-2 reversed phase column using the mixture of acetonilrile, water and phosphate buffer （10：80：10, by vol） as mobile phase and detected by UV detector at 250 nm. In addition, the results were quantitated by external standard method. [ Results ] The results showed that the standard deviations of pymetrozine and nitenpyram were 0.29 and 0.22, the coefficients of variation were 0.51 and 1.21%, the average recoveries were 100.7 and 99.1%, the linear correlation coefficients were 0.9995 and 0.9998, respectively. [Conclusions ] The method has the advantages of convenient, good separation, high precision and accuracy and good linear relation.
出处
《农药》
CAS
CSCD
北大核心
2017年第1期38-40,共3页
Agrochemicals
