摘要
碱性条件下,金纳米对鲁米诺-铁氰化钾化学发光体系有增敏作用,克拉维酸钾可以显著增强该化学发光体系的发光信号。基于此,在优化化学发光反应条件的基础上,结合流动注射技术,提出了测定克拉维酸钾的新方法,并对其可能的化学发光机理进行了探讨。测定克拉维酸钾的线性范围为0.01~1.0μg/m L,相关系数(r)为0.9964,检出限为6.9 ng/m L,相对标准偏差(RSD)为1.1%(0.6μg/m L克拉维酸钾溶液,n=11)。方法可用于牛奶中克拉维酸钾的测定,回收率为96.0%~99.7%。
It was found that gold nanoparticles （AuNPs） could enhance the chemiluminescence （CL） of the Luminol-K3 [ Fe （ CN）6 ] system under alkaline condition. The CL of Luminol-K3 [ Fe （CN） 6 ]＂ AuNPs system is strongly enhanced in the presence of elavulanate potassium. Based on this, a novel CL method was developed for determining clavulanate potassium. The experimental conditions were optimized by flow-injection method. The possible CL mechanism was also discussed. Under the optimal conditions, the relative CL intensity was linearly proportional to concentration of clavulanate potassium in the range of 0. 01 - 1.0 μg/mL with a correlation coefficient of 0. 9964 and a detection limit of 6.9 ×10^-9 g/mL. The RSD （n = 11） was 1.1% for 0. 6 μg/mL clavulanate potassium. The proposed method was successfully applied to analyze clavulanate potassium in milk samples with recoveries of 96. 0% -99. 7%.
出处
《分析试验室》
CAS
CSCD
北大核心
2017年第1期100-104,共5页
Chinese Journal of Analysis Laboratory
关键词
克拉维酸钾
金纳米
鲁米诺
化学发光
Clavulanate potassium
Gold nanoparticles
Luminol
Chemiluminesenee
