摘要
研究发现,在金纳米粒子存在下,异烟肼与鲁米诺反应可产生强的化学发光.采用响应曲面优化法中的Box-Behnken设计,对试剂浓度进行了优化.在优化的实验条件下,相对化学发光强度与异烟肼的质量浓度在5.0×10-9～3.0×10-7 g/mL范围内呈线性关系.方法的检出限为4.1×10-9 g/mL异烟肼,相对标准偏差为1.3%(1.0×10-7 g/mL异烟肼溶液,n=11).该方法已用于片剂、注射液和人血清中异烟肼含量的测定.同时,对化学发光反应的可能机理也进行了讨论.
It was found that strong chemiluminescence（CL） was directly produced by the reaction of isoniazid with luminol in the presence of gold nanoparticles（AuNPs） in an alkaline solution.Based on this finding,a simple and sensitive CL method has been developed for the determination of isoniazid.The re-agent concentrations,including luminol concentration,NaOH concentration and AuNPs concentration,were optimized with the aid of Box-Behnken design and response surface methodology.The method allows the measurement of 5.0×10-9～3.0×10-7 g/mL isoniazid with a detection limit of 4.1×10-9 g/mL.The rela-tive standard deviation is 1.3% for 1.0×10-7 g/mL isonazid solution in 11 repeated measurements.The proposed method was applied to the analysis of isoniazid in tablets,injections and in spiked human blood serums.The possible CL reaction mechanism was also discussed by the study of the UV-vis spectrum,the CL spectrum,and the effect of dissolved oxygen and reactive oxygen species scavengers on the reaction.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2011年第6期745-751,共7页
Acta Chimica Sinica
基金
中央高校基本科研业务费专项资金(No.GK200902012)
陕西师范大学优秀科技预研(No.200902016)资助项目
关键词
异烟肼
化学发光
金纳米粒子
响应曲面优化法
isoniazid
chemiluminescence
gold nanoparticles
response surface methodology
