摘要
为了研究从水蓼中分离得到的11个化合物的抑菌活性及其中部分化合物的抗氧化活性,采用96孔板二倍稀释法进行抑菌活性测试,以最小抑菌浓度(MIC)考察各化合物的抑菌能力强弱;通过DPPH法测定自由基清除率,利用origin 70拟合出标准曲线,计算各化合物的IC50,以此反映其抗氧化能力大小.实验结果显示从水蓼中分离出的11个化合物对各细菌表现出不同程度的抑制作用,其中黄酮类化合物的抑菌效果较好,槲皮素对金黄色葡萄球菌的抑制作用最好,最小抑菌浓度为31.3μg·mL^(-1);所检测的化合物均表现出良好的抗氧化活性,其中槲皮素、没食子酸和鞣花酸的抗氧化能力强于Vc.结果表明从水蓼中分离得到化合物有良好的抑菌和抗氧化活性,其中黄酮类化合物的活性强弱因其结构的不同而表现出明显的差异,烯醇式结构较多的化合物表现出较强的抗氧化能力.
To study on the antibacterial activity of the 11 compounds isolated from Polygo-num hydropiper L. and some compounds7 antioxidant activity was measured by microdilu-tion method on 96-well plates. The minimum inhibiting concentration （MIC） was used for e-valuating the degree of antibacterial ability of each compound. The antioxidant capacity was measured by the DPPH radical-scavenging rate. The curves were plotted and the IC50 of com-pounds was determined by using origin 70 software,with IC50 expressing antioxidant capacity of different compounds. The results showed that the 11 compounds from P o l y g o n u m h y -dropiper L. manifested different degree of antibacterial activity,and the flavonoids had good antibacterial effect quercetin showed the best activity to restrain the growth of the Staphy-lococcus aureus with MIC value of 31. 3 tug mL-1. The compounds detected showed good antioxidant activity,and the antioxidant effect of quercetin,gallic acid and ellagic acid are better than vitamin C. Results indicate that these compounds isolated from P o ly g o n um h y -dropiper L. have good antibacterial and antioxidant activity. The different activity of flavonoids is because of their different structures. The more enol structure in the flavonoids,the stronger antioxidant capacity it has.
作者
马养民
李梦云
郭林新
乔珂
范超
MA Yang-min LI Meng-yun GUO Lin-xin QIAO Ke FAN Chao(College of Chemistry and Chemical Engineering, Key Laboratory of Auxiliary Chemistry 8/Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science Technology, Xi^an 710021, China)
出处
《陕西科技大学学报（自然科学版）》
2017年第1期120-123,138,共5页
Journal of Shaanxi University of Science & Technology
基金
国家公益性行业(林业)科研专项项目(200904004)
关键词
水蓼
化学成分
抑菌活性
抗氧化活性
构效关系
Polygonum hydropiper L.
chemical compounds
antibacterial activity
antioxidant activity
QSAR
