摘要
【目的】通过对铜/碳-核/壳纳米颗粒(CCCSNs)在毛竹、富贵竹和陆地棉3种植物中积累分散效果的研究,以期为利用CCCSNs提高植物材料防腐能力提供依据。【方法】试验用0,0.1和0.5 g·L-1CCCSNs水培毛竹和富贵竹,以每盆0,0.1和0.5 g CCCSNs土培陆地棉。自来水浇灌、常规管理,50天后一次性破坏性取样,用原子吸收光谱分段测量3种植株体内铜含量,并对根系细胞进行透射电镜和能谱扫描观测。【结果】CCCSNs可以从根系进入植物体向地上部传输,但植物积累CCCSNs的量与物种有关。0.1和0.5 g·L-1处理的毛竹地上部铜含量分别为13.19和11.79μg·g^(-1),比对照提高263%和225%;富贵竹地上部铜含量分别为29.31和27.95μg·g^(-1),比对照提高104%和90%;陆地棉地上部铜含量分别为5.22和6.53μg·g^(-1),比对照提高了24%和52%。地上部最高铜含量毛竹为21.65μg·g^(-1),富贵竹为44.88μg·g^(-1),陆地棉为9.19μg·g^(-1)。3种植物中地上部平均绝对铜含量和最高铜含量均为富贵竹最高;但与对照相比提高的百分率以毛竹为最高。透射电镜和能谱扫描发现,经过CCCSNs处理的植株,CCCSNs可以积聚在其根细胞的细胞质内、细胞膜内侧、细胞壁和细胞间隙中;3种植物之间根细胞积累CCCSNs的方式没有明显差别。【结论】将CCCSNs施用毛竹、富贵竹和陆地棉根部时,CCCSNs可以通过根系吸收并向地上部(茎和叶)运输,运输能力与植物种类有关。植物积累CCCSNs的量在低浓度时与施用量正相关,高浓度时与施用量没有稳定的关系。此外,CCCSNs可以积聚在3种植物根细胞的细胞质内、细胞膜内侧、细胞壁和细胞间隙内。虽然该试验已经证明CCCSNs可以从根系进入植物体内,但是CCCSNs如何从细胞间隙进入细胞和以何种方式运送至地上部等问题仍需要进一步研究。
【Objective】CCCSNs is a new renewable composite nano-material that is called as the Copper-Carbon CoreShell Nanoparticles,and it is fabricated by using cotton fibers as the template,with copper atoms homogeneously embedded in carbon black as a composite material. The special carbon shells can protect the copper core from oxidation,by which the Cu shows the typical antibacterial,antifungal,antifouling properties. This paper is expected to explore the the accumulation and dispersion of CCCSNs in Phyllostachys edulis,Dracaena sanderiana and Gossypium hirsutum,in order to provide the basis for the plants to make use of CCCSNs in promoting the anti-corrosion ability. 【Method】The P.edulis and D. sanderiana were grown in hydroponic media with adding 0,0. 1 or 0. 5 g L- 1CCCSNs. G. hirsutum was grown in potting soil amended with 0,0. 1 or 0. 5 g CCCSNs per a pot,and watered with tap water. The three plant species were managed regularly. After 50 days,we harvested all samples that were used to test the copper concentration which could be considered as CCCSNs signal. The samples were dissolved by hydrogen nitrate and the extract was measured with atomic absorption spectrum. The anatomy of roots was observed by TEM and DES. 【Results】CCCSNs was able to enter a plant and transport from roots to stems and leaves,and the accumulation of CCCSNs was dependent on the different species. The Cu concentration of P. edulis stems and leaves,when their roots exposed to 0. 1 and 0. 5 g·L- 1CCCSNs,was 13. 19 and 11. 79 μg·g-1,and were 263% and 225% higher than that of the control,respectively. With0. 1 and 0. 5 g·L- 1CCCSNs treatment,the copper concentration of D. sanderiana stems and leaves was 29. 31 and 27. 95μg·g-1and 104% and 90% higher than that of the control,respectively. The Cu concentration in stems and leaves of G.hirsutum treated with 0. 1 and 0. 5 g·pot- 1was 5. 22 and 6. 53 μg·g-1and 24% and 52% higher than that of the control.The highest Cu concentration in stems and leaves of P. edulis,D. sanderiana and
出处
《林业科学》
EI
CAS
CSCD
北大核心
2016年第11期47-54,共8页
Scientia Silvae Sinicae
基金
国家林业局948项目(2013-4-23)
浙江省科技计划项目(2014F10047)
关键词
金属纳米颗粒CCCSNs
积累
分布
毛竹
富贵竹
陆地棉
metal nanoparticles CCCSNs
accumulation
allocation
Phyllostachys edulis
Dracaena sanderiana
Gossypium hirsutum
