摘要
本研究以海陆渐渗系13-1与陆地棉辽棉12构建的F2群体为材料,利用4 500对中具有多态性的84对SSR引物进行群体间分析。研究结果显示:在p<0.05显著水平上,共有19个分子标记表现偏分离,占总标记数的25.3%,其中有8个标记偏向父本辽棉12,占偏分离标记总数的42.1%;10个标记偏向母本13-1,占偏分离标记总数的52.63%;1个标记偏向杂合体,占偏分离标记总数的5.27%。这些标记在图谱上有两种分布形式,分别为成簇分布和孤立分布。在7条不同的染色体上均发现偏分离标记,其中在1染色体上发现1个热点区域。本研究分析了产生偏分离的原因,并讨论偏分离标记对QTL定位的影响。
F-_2 materials derived from a cross between introgression lines 13-1×Liaomian 12 were used as mapping population in cotton. Among the 84 polymorphic markers analyzed, 19 markers(25.3%) showed the genetic distortion(p<0.05) in the F2 population. Among these segregation distortion SSR markers, 8 SSR marker alleles were distorted to the male parent Liao12(42.1%), 10 SSR markers allels were distorted to the female parent 13-1(52.61%), and one SSR marker alleles were distorted to the heterozygote(5.27%). These segregation distortion SSR markers were in cluster of tightly loci or single marker, which distributed in 7 different chromosomes with hot region in one of them. In this research, reasons for segregation distortion and effects of segregation distortion on genetic mapping and QTL analysis were discussed.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第2期424-430,共7页
Molecular Plant Breeding
基金
国家重点基础研究发展计划(2012CB722205)
国家自然科学基金(31360265)
石河子大学动植物育种专项(gxjs2013-y203)
石河子大学高层次人才科研启动项目(RCZX201313)共同资助
关键词
棉花
海陆渐渗系
SSR分子标记
偏分离
遗传分析
Cotton
Introgression lines
SSR Marker
Segregation distortion
Genetic analysis
