摘要
分枝数是影响大豆产量的重要因素之一,与植株结荚率直接相关;同时也是决定大豆株型的重要组成因子,并通过调节群体结构、种植密度等进一步影响产量。目前关于大豆分枝数QTL(quantitative trait loci)精细定位与图位克隆的报道极少。因此,发掘参与调控大豆分枝的基因/QTL对于株型建成的基础研究和高产品种培育的应用研究都具有重要意义。本研究在少分枝品种垦丰19(KF19)与多分枝品种垦农24(KN24)组合F2的基础上,培育出由606个株系组成的F7:8重组自交系(recombinant inbred lines,RIL)群体,以及由1486个单株KF19-BC3F2和1150个单株KN24-BC2F2组成的2个回交群体。在18号染色体分枝数QTL新位点(qBN-18)的定位区间内筛选出多态性SSR标记11个,利用RIL群体将qBN-18的定位区间由1.6 Mb缩小到113 kb。在定位区间内开发了2个InDel标记BR69与BR77。进一步利用回交群体筛选交换单株,将qBN-18定位区间缩小到63.7 kb,包括9个基因。本研究结果为大豆分枝数的基因图位克隆及分子标记辅助育种创造了条件。
The branch number is one of the important factors influencing soybean yield,which is directly related to pod setting rate.At the same time,it is also an important component of soybean plant type,and further affects the yield by adjusting the population structure and planting density.At present,there is few report related to map-based cloning of genes related to branch number.Therefore,the discovery of genes/QTL involved in the regulation of soybean branching is of great significance for the basic research on the establishment of plant type and the applied research on the development of high-yielding varieties.In this study,based on the F2 of crossing low-branched variety Kenfeng 19(KF19)and high-branched variety Kennong 24(KN24),we developed the F7:8 recombinant inbred line(RIL)population,consisting of 606 lines,and two backcrossing populations consisting of 1486 individuals for KF19-BC3F2 and 1150 individuals for KN24-BC2F2.Within the localization interval of the new QTL of the branch number of chromosome 18(qBN-18),11 polymorphism SSR markers were screened out to identify the RIL population,and region of qBN-18 was reduced from 1.6 Mb to 113 kb.After developing two InDel markers BR69 and BR77 in the mapping region,the backcross population was used to screen the exchange individuals,the interval of qBN-18 was further reduced to 63.7 kb,including 9 genes.Those results provide the information for gene map-based cloning and molecular marker assisted breeding of branch number in soybean.
作者
吴海涛
张勇
苏伯鸿
Lamlom F Sobhi
邱丽娟
WU Hai-Tao;ZHANG Yong;SU Bo-Hong;Lamlom F Sobhi;QIU Li-Juan(College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;Keshan Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161606,Heilongjiang,China;National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization,Ministry of Agriculture/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Plant Production Department,Faculty of Agriculture Saba Basha,Alexandria University,Alexandria,Egypt)
出处
《作物学报》
CAS
CSCD
北大核心
2020年第11期1667-1677,共11页
Acta Agronomica Sinica
基金
“十三五”国家重点研发计划项目(2016YFD0100201)
中国农业科学院科技创新工程和大豆种质资源保护与利用项目(2019NWB036-05)资助.
关键词
大豆
分枝数
QTL定位
候选基因
soybean
branch number
QTL mapping
candidate genes
