摘要
株高和一次有效分枝高度是与甘蓝型油菜结荚层厚度、收获指数紧密关联的重要农艺性状,有关株高的数量性状位点(quantitative trait locus,QTL)和全基因组关联分析(genome-wide association study,GWAS)已有很多报道,但对一次有效分枝高度的QTL和GWAS定位以及候选基因筛选的研究报道较少。本研究利用已构建的高密度遗传连锁图对2016和2017年2个环境的186个株系组成的重组自交系群体株高和一次有效分枝高度及其最佳线性无偏预测(best linear unbiased prediction,BLUP)值进行QTL定位共检测到8个株高的QTL,分别位于A03、A04和A09染色体,单个QTL解释4.60%~13.29%的表型变异,其中位于A04染色体上的QTL(q-2017PH-A04-2和q-BLUP-PH-A04-2)在2017年和BLUP中均被检测到;检测到9个一次有效分枝高度QTL,分别位于A01、A02、A05、A09、C01和C05染色体上,单个QTL解释5.12%~19.10%的表型变异,其中q-2017BH-A09-1、q-BLUP-BH-A09-2和q-BLUP-BH-A09-3有重叠区段。同时,利用课题组前期完成的588份重测序自然群体进行全基因组关联分析,2年共检测到与株高显著关联的50个SNP位点和与一次有效分枝高度显著关联的12个SNP位点;根据SNP的物理位置,筛选出参与细胞增殖、细胞扩增、细胞周期和细胞壁活动的13个株高候选基因,以及参与赤霉素、亚精胺等合成代谢途径、核糖体组成和在光合、萌发等过程中有一定作用的一次分枝高度的11个候选基因,并利用荧光定量PCR技术验证候选基因在极端材料中的表达情况。本研究结果将为油菜株型改良及后续基因的功能研究提供理论依据。
Plant height(PH)and the first branch height(BH)are important agronomic traits closely related to thickness of pod canopy and harvest index of Brassica napus.There are many reports on quantitative trait locus(QTL)and genome-wide association study(GWAS)for PH,but few reports on QTL and GWAS localization and candidate gene screening for BH.In this study,QTLs for PH and BH using the 186 recombinant inbred lines(RIL)in the two environments of 2016 and 2017 were detected based on the high-density genetic linkage map.A total of eight PH QTLs located on chromosomes A03,A04,and A09 were detected in the two environments of 2016 and 2019,with the explained phenotypic variation of individual QTL range from 4.60%to 13.29%,among which overlapped QTLs(q-2017PH-A04-2 and q-BLUP-PH-A04-2)were detected both in 2017 and based on Best Linear Unbiased Prediction(BLUP).Nine BH QTLs located on chromosomes A01,A02,A05,A09,C01,and C05 were detected,in which a single QTL explained 5.12% to 19.10% of phenotypic variation.Among them,q-2017BH-A09-1,q-BLUP-BH-A09-2,and q-BLUP-BH-A09-3 were overlapped.GWAS for PH and BH was performed using 588 resequencing natural populations established by our previous study.A total of 50 SNPs associated with PH and 12 SNPs associated with BH were detected in two years.Thirteen PH candidate genes involved in cell proliferation,cell multiplication,cell cycle and cell wall activity and eleven BH candidate genes involved in the synthesis and metabolism of gibberellin and spermidine,ribosome composition,photosynthesis and germination were screened based on the physical locations of SNPs,and their expressions in extreme phenotypes by qRT-PCR.This result will provide a theoretical basis for improving plant architecture and subsequent functional studies of genes.
作者
霍强
杨鸿
陈志友
荐红举
曲存民
卢坤
李加纳
HUO Qiang;YANG Hong;CHEN Zhi-You;JIAN Hong-Ju;QU Cun-Min;LU Kun;LI Jia-Na(College of Agronomy and Biotechnology,Southwest University/Chongqing Engineering Research Center for Rapeseed,Chongqing 400715,China;Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China)
出处
《作物学报》
CAS
CSCD
北大核心
2020年第2期214-227,共14页
Acta Agronomica Sinica
基金
重庆市民生工程主题专项(cstc2016shms-ztzx80020)
国家自然科学基金-云南联合基金项目(U1302266)
国家重点研发计划项目(2018YFD0100504-05)资助~~
