摘要
以优良玉米(Zea maysL.)自交系抗感杂交组合(R15×掖478)的F2群体为材料,构建了239个分子标记(包括151个SSR标记和88个AFLP标记)的玉米分子连锁图,覆盖全基因组3 463.5 cM,相邻标记间的平均间距为14.5 cM。在239个标记中,16个SSR标记和9个AFLP标记表现偏分离(P<0.05)。在4条不同的染色体上发现5个偏分离的热点区域,其中3个与已经定位的配子体基因的位置相近,表明配子体基因可能是导致偏分离的部分原因。还发现SDR2和SDR7可能是2个偏分离的新热点区域。进一步讨论了引起偏分离的原因,以及偏分离标记对估算遗传距离的影响。当存在单个的配子体基因时,遗传距离的估算不受偏分离的影响;当存在2个或者2个以上配子体基因时,对遗传距离的估算有偏差。
230 F2 individuals derived from a single cross between inbred maize （Zea mays L. ） lines R15 （resistant） and Ye 478 （susceptible） were used to construct a genetic linkage map. A total of 239 newly screened markers including 88 AFLP markers and 151 SSR markers were mapped on the 10 chromosomes covering 3 463.5 cM of maize genome, with the average interval of 14.5 cM. Among the 239 markers, 16 SSR markers （10.6%） and 9 AFLP markers（8.9%）showed genetic distortion （P 〈 0.05）. Five putative segregation distortion regions （SDRs） were detected on 4 chromosomes, including 2 new ones （SDR2 and SDR7 ）. The other 3 SDRs were located near the regions where gametophyte genes were mapped, indicating that segregation distortion could be partially caused by gametophytic factors.
出处
《作物学报》
CAS
CSCD
北大核心
2006年第9期1391-1396,共6页
Acta Agronomica Sinica
基金
SupportedbyProgramforChangjiangScholarsandInnovativeResearchTeaminUniversity(IRT0453),theNationalNaturalScienceFoundationofChina(30370889),theNationalHighTechnologyResearchandDevelopmentProgramofChina(2003AA207070)andSichuanProvincialBiologicalKeyProject
