摘要
为了探究棉花群体中SSR分子标记的偏分离现象,以本课题构建的两个陆地棉群体(‘冀棉11’ב中植棉2号’) F_2和(‘常抗棉’בTM-1’) RIL群体为研究材料,利用一万余对SSR引物同时对其双亲进行多态性引物筛选,分别获得133个在F_2亲本间具有多态的SSR标记,119个在RIL亲本间具有多态的SSR标记,以此为基础构建连锁图谱,对进入F_2连锁图谱的114个多态性标记以及进入RIL连锁图谱的78个多态性标记进行偏分离卡方检测,对比分析后发现:RIL群体的标记偏分离率远高于F_2群体,偏分离率分别为60.26%和19.30%。同时,我们对两个图谱中的共有标记及其所在染色体进行了比对分析,认为在染色体D01 (Chr15)和D03 (Chr17)上可能存在导致偏分离的配子基因。本研究为其它连锁图谱的构建及QTL的准确定位提供帮助,并将有助于定位出棉花中导致偏分离的配子体基因。
In order to explore the phenomenon of segregation distortion of SSR molecular markers in cotton population, two upland cotton populations （‘Zhongzhi cotton 2＇בJi cotton 11＇） F2 and （＇Chang kang cotton＇בTM-1＇） R1L population constructed in this topic were used as research materials. More than ten thousand SSR primers were used to select polymorphic primers for their parents at the same time, 133 polymorphic SSR markers between F2 parents and 119 polymorphic SSR markers between RIL parents were obtained respectively. Based on this, the linkage map was constructed, 114 polymorphic markers entering F2 linkage map and 78 polymorphic markers entering RIL linkage map were detected by separation distortion chi square test. After comparative analysis, we found the segregation distortion rate of RIL population was much higher than that of F2 population, and the separation distortion rates were 60.26% and 19.30%, respectively. At the same time, we compared and analyzed the common markers and their chromosomes in the two maps, and considered that there might be gamete genes that lead to segregation distortion on chromosomes D01 （Chr15） and D03 （Chr17）. This study would provide help for the construction of other linkage maps and the accurate positioning of QTL, and would help to locate the gametophyte genes that lead to segregation distortion in cotton.
作者
鲁宁宁
张凯
赵云雷
陈伟
赵佩
龚海燕
崔艳利
桑晓慧
王红梅
Lu Ningning;Zhang Kai;Zhao Yunlei;Chen Wei;Zhao Pei;Gong Haiyan;Cui Yanli;Sang Xiaohui;Wang Hongmei(Cotton Research Institute of Chinese Academy of Agricultural Sciences,State Key Laboratory of Cotton Biology,Anyang,45500)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第19期6372-6379,共8页
Molecular Plant Breeding
基金
中国农业科学院2017创新工程基金项目(CAAS-ASTIP-2017-ICR)资助
