摘要
目的探讨阴道病学妇女宫颈人乳头瘤病毒(human papillomavirus,HPV)感染及基因型分布特征。方法采用PCR-反向点杂交法对解放军第九五医院816例宫颈病变妇女宫颈脱落细胞进行HPV检测,并进行23种HPV基因分型鉴别。结果本组816例接受HPV分型检测中,HPV阳性532例(65.20%),其中371例(69.74%)高危型HPV(HR-HPV)感染,161例(30.26%)低危型HPV(LR-HPV)感染。45岁以下人群HPV感染率较高;宫颈癌患者HPV感染率为97.10%高于CINⅡ-Ⅲ患者(80.54%)、CINⅠ患者(41.55%)、宫颈炎患者(17.20%),差异具有统计学意义(P<0.05);HPV感染亚型排列前5位分别为HPV16、58、18、11、56型。单一感染几率为79.70%,重复感染率为20.30%;单一感染中以HR-HPV感染率为主,多重感染以双重感染为主。结论 44岁以下女性HPV感染的高危人群,HPV感染以16、58、18、11、56为主,宫颈癌及癌前病变患者HPV感染率较高,重视HPV感染筛查对防治宫颈癌有积极意义。
Objective To study cervical human papillomavirus（ HPV） infection and genotype distribution characteristics in patients with vaginal disease. Methods The PCR dot blot was used to carry out HPV detection of cervical exfoliated cells in 816 women with cervical lesions in the hospital,and 23 kinds of HPV genotypes were identified. Results Among the 816 cases receiving HPV type detection,HPV of 532 cases（ 65. 20%） was positive,including 371 cases（ 69. 74%） with low-risk HPV（ HR-HPV） infection,161 cases（ 30. 26%） with low-risk HPV infection（ LR-HPV）（ P〈0. 05）. The HPV infection rate below 45 years old was relatively higher. The HPV infection rate in patients with cervical cancer（ 97. 10%） was higher than that in patients with cervical intraepithelial neoplasia（ CIN） II- III（ 80. 54%）,patients with CIN Ⅰ（ 41. 55%） and patients with cervicitis（ 17. 20%）（ P〈0. 05）. The top five types of HPV infection were HPV16,58,18,11 and 56 type,respectively. The single infection rate（ 79. 70%）was higher than multiple infection rate（ 20. 30%）（ P〈0. 05）; HR-HPV infection was the main infection in single infection,and double infection was the main infection in multiple infection. Conclusion Below 45 years old women are the high-risk groups of HPV infection. 16,58,18,11 and 56 are the main types of HPV infection. The infection rates of patients with cervical cancer and precancerous lesions are relatively higher. Paying attention to the screening of HPV infection has positive significance in the prevention and treatment of cervical cancer.
出处
《中国生化药物杂志》
CAS
2016年第11期183-185,共3页
Chinese Journal of Biochemical Pharmaceutics
