摘要
目的研究假酸浆Nicandra physaloides果中的黄酮类化学成分及其免疫活性。方法采用硅胶、ODS及HPLC等色谱方法分离化合物，利用NMR、MS波谱学方法鉴定其结构，并采用脂多糖（LPS）诱导的小鼠脾淋巴细胞增殖模型评价化合物的免疫活性。结果从假酸浆果70％乙醇回流提取物的正丁醇部位分离得到13个化合物，分别鉴定为3＂-hydroxy-puerarin（1）、puerarin-6＂-O-glucoside（2）、daidzein 8-C-[α-D-apiofranosyl-（1→6）]-β-glucopyranoside（3）、葛根素（4）、3＂-methoxyl-puerarin（5）、6-C-α-L-arabinosyl-8-C-β-D-glycosylchrysin（6）、oroxylin A-7-O-β-D-glucuronidemethylester（7）、大豆黄苷（8）、黄芩苷（9）、quercetin-3-O-β-D-galactopyranoside（10）、quercetin 3-rutinoside（11）、kaempferol 3-rutinoside（12）和quercetin 3-glucoside（13）；化合物对LPS刺激的小鼠脾淋巴细胞增殖影响的实验表明，化合物1～3、7、9、10、12、13显示出对小鼠脾淋巴细胞增殖的抑制作用，而化合物6表现出对小鼠脾淋巴细胞增殖的促进作用。结论化合物1～7为首次从茄科植物中分离得到，8～13为首次从该属植物中分离得到，且化合物l～3、6、7、9、10、12、13可能具有潜在的免疫活性。
Objective To investigate the flavonoids from the fruits of Nicandra physaloides and clarify the immune activities of the isolated compounds. Methods The separations and purifications were taken by silica gel and ODS chromatogram columns as well as preparative HPLC, and the structural identification based on physicochemical property, 1H-NMR, and 13C-NMR as well as HR-MS data. And the immune activities were evaluated by mice splenic lymphocyte proliferation model induced by LPS. Results Thirteen compounds were obtained from the butanol fraction of the 70% ethanol extract ofN. physaloides fruits, which were identified as 3″-hydroxy-puerarin （1）, puerarin-6″-O-glucoside （2）, daidzein 8-C-[α-D-apioffanosyl-（1→6）]-β-D-glucopyranoside （3）, puerarin （4）, Y＇-methoxyl-puerarin （5）, chrysin 6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside （6）, oroxylin A-7-O-β-D-glucuronide methyl ester （7）, daidzin （8）, baicalin （9）, quercetin-3-O-β-D-galactopyranoside （10）, quercetin 3-rutinoside （11）, kaempferol 3-mtinoside （12）, and quercetin 3-glucoside （13）, respectively. The results indicated that compounds 1--3, 7, 9--10, and 12--13 could inhibit the proliferation of mice spleen lymphocytes and 6 could promote the proliferation in the influence of mice spleen lymphocytes proliferation induced by LPS. Conclusion Compounds 1--7 are isolated from the plants of Solanaceae for the first time, compounds 8--13 are obtained from the plants ofNicandra Adans. firstly. And the compounds 1--3, 6, 7, 9--10, and 12--13 possibly possess the potential immune activities.
出处
《中草药》
CAS
CSCD
北大核心
2016年第22期3965-3969,共5页
Chinese Traditional and Herbal Drugs
