摘要
采用PCR-RFLP方法对烟台黑猪DQB基因外显子2的多态性进行研究,并对该位点与仔猪腹泻的关联进行了分析。结果表明,在烟台黑猪DQB基因外显子2上共检测出4种等位基因(A、B、C、D),7种RFLP带型(AA、BB、CC、AB、AC、BC、AD),该位点具有较丰富的多态性。卡方适合性检验显示,烟台黑猪的DQB基因外显子2没有达到Hardy-Weinberg平衡(P<0.05)。最小二乘法分析结果表明,烟台黑猪的性别和DQB外显子2的基因型均对仔猪腹泻无显著影响(P>0.05),而性别与基因型的互作效应对仔猪腹泻具有极显著的影响(P<0.01),在公猪群体中,AD基因型仔猪腹泻评分高于AA基因型个体(P<0.05),在母猪群体中BB、AC、BC、CC基因型仔猪腹泻评分高于其他基因型仔猪(P<0.05)。
Polymorphism of SLA-DQB exon 2 in Yantai black pigs was studied using PCR-RFLP,and its correlation with piglets diarrhea was analyzed. The results showed that there were four alleles（ A,B,C,D） and seven RFLP genotypes（ AA,BB,CC,AB,AC,BC,AD）,polymorphism of the DQB exon 2 was abundant. The chi-square test displayed DQB exon 2 of Yantai black pigs deviated Hardy-Weinberg equilibrium（ P〈0. 05）. The results of least squares showed that the sex of Yantai black pig and genotype of DQB exon 2 did not have significant influence on diarrhea piglets（ P〉0. 05）. However,polymorphism of DQB exon 2 in different genders affected diarrhea piglets（ P〈0. 01）. In males,diarrhea score of genotype AD was higher than that of AA genotype（ P〈0. 05）,in female,scores of BB,AC,BC,CC were higher than others（ P〈0. 05）.
出处
《浙江农业学报》
CSCD
北大核心
2016年第10期1671-1677,共7页
Acta Agriculturae Zhejiangensis
基金
中央高校基本科研业务费专项资金本科生项目(107422015033)
西北民族大学中央高校基本科研业务费专项资金资助项目(31920130048)
西北民族大学引进人才项目(xbmuyjrc201408)
