摘要
通过PCR—SSCP技术检测了7个品种300匹马ELA—DQA＊^exon2的多态性。用12％非变性聚丙烯酰胺凝胶电泳将已变性的PCR扩增产物分离，银染法显色。结果300匹马中共出现27种基因型：10种纯合子和17种杂合子；7个品种马的He和PIC值均表现为高度多态；等位基因序列分析得到10个等位基因间的Kimura双参数遗传距离为0．015～O．147，符合（dN：〉dS）的正选择检验。因此表明马的ELA—DQA＊^exon2多态性丰富。
ELA-DQA＊^exon2 locus was used to detect 300 horses of 7 breeds. The PCR amplified products of the loci were detected by 12 percent non-denatured polyacry lamide gel electrophoresis and showing in silver staining protocol. The results showed that 27 genotypes were detected in 300 horses： 10 homozygotes and 17 heterozygotes; The values of He and PIC represent highest polymorphism; Kimura 2-parameter genentic distances between 10 alleles were from 0. 015 to 0. 147 and accorded Z-test（dN〉dS）by sequense analysis. So horses had abundant polymorphism in ELA-DQH ＊^exon2 locus.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2006年第10期951-955,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
内蒙古自治区高等学校科学研究资助项目(NJ03093)
