摘要
目的:研究NaNO_2对雄性小鼠的损伤及修复机制。方法:昆明种雄性小鼠,剂量组经口给予NaNO_2(15、30、60 mg/(kg·d)),对照组给予等体积生理盐水,连续14 d,末次染毒24 h后,处死各剂量组1/3小鼠,余下小鼠分两组,给予NaNO_2等剂量枸杞多糖和等体积生理盐水,连续14 d,处死全部实验动物。测定主要脏器、睾丸及附睾的重量、脏器系数,血清睾酮水平和精子畸形率。结果:随NaNO_2剂量增加,睾酮水平、脾脏重量均下降(P<0.05),精子畸形率升高(P<0.05);停止给予NaNO_2后,在15、30mg/kg NaNO_2组,精子畸形率下降,睾酮水平升高及脾脏器系数,与之前比较差异具有统计学意义(P<0.05),其他指标差异无统计学意义。结论:NaNO_2具有抑制小鼠睾酮水平、诱导精子畸变的能力;停止染毒后,小鼠自身具有一定修复能力,枸杞多糖也可能具有一定的修复作用,但还有待进一步研究。
Objective： To study the sodium nitrite injury and repair mechanisms to the male mice. Methods： Kunming species male mice were used in the experiment. Dose groups were administered by oral with NaNO2（15, 30, 60 mg/（kg·d））, while the control groups had been given normal saline of the same volume for 14 days. After 24 hours of the last exposure to NaNO2, one-third of mice in three dose groups were killed. The remaining mice were divided into two groups. One group was given Lyceum barbarum polysaccharide, while another group was given normal saline of the same volume for 14 days and then killed all the mice. Experiment measured index included the main organ weight, organ coefficient, serum testosterone level and sperm deformity rate. Results： With the increasing dose of NaNO2, the mice serum testosterone level and the spleen weight were decreased significantly（all P value〈0.05）, the sperm deformity rate was increased significantly（all P value〈0.05）. After stopping giving NaNO2, the level of serum testosterone were increased, the sperm deformity rate and spleen organ coefficient were declined in 15, 30 mg/（kg·d）NaNO2 groups,the three indexes were statistically significant comparing with previous experiments（all P value〈0.05）. The other indexes had no change. Conclusion： NaNO2 had the ability to inhibit the level of serum testosterone and induce the sperm deformity in mice. After stopping exposing to NaNO2, the mice can repair some damages by themselves, the Lyceum barbarum polysaccharide may also have recovery effect, which needs further study.
出处
《大理大学学报》
CAS
2016年第10期35-38,共4页
Journal of Dali University
基金
大理大学开放实验室基金资助项目(2014-79)
大理大学大学生科研基金资助项目(KYSX2013013)
