摘要
目的研究枸杞多糖（LBP）对正常人外周血巨噬细胞功能的影响。方法无菌分离人外周血单核细胞,加入植物血凝素（PHA）和不同质量浓度的LBP,用MTT法检测巨噬细胞对肝癌细胞株Bel-7402生长的影响,ELISA法检测LBP作用后巨噬细胞培养上清液中肿瘤坏死因子-α（TNF-α）水平。结果用LBP处理后的巨噬细胞对肿瘤细胞的杀伤功能增强,80、100μg/mLLBP刺激后巨噬细胞吞噬作用明显增强（P〈0.01）。80、100μg/mLLBP组肿瘤细胞与巨噬细胞培养上清液中TNF-α水平明显增高（P〈0.01）,证实该作用可能与LBP促进巨噬细胞分泌TNF-α增多有关。结论 LBP能增强人外周血巨噬细胞免疫功能,发挥抗肿瘤作用。
Objective To study effect of lycium bararum polysaccharides（LBP）on function of human peripheral blood macrophages.Methods Macrophages from human peripheral blood were isolated and coincubated with phytohemagglutin（PHA）and different concentration of LBP.The effect of LBP on the phagocytosis of macrophage to the growth of human hepatocarcinoma cells Bel7402 were measured by MTT method.TNF-αsecretion in supernatant of macrophage culture after LBP treatment were determined with ELISA assay.Results LBP could strengthen the macrophage to inhibit the proliferation of hepatocarcinoma cells,The proliferation of hepatocarcinoma cells was inhibited significantly by 80,100μg/mL LBP respectively（P〈0.01）.The secretion of TNF-α in supernatant of tumour cell and macrophages culture after LBP treatment was enhanced by 80,100μg/mL LBP significantly（P 0.01）.which may be related with TNF-αproduction.Conclusion LBP could regulate the immune response of macrophage from human peripheral blood,which may be related with killing carcinoma cells.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第16期2177-2178,共2页
Chongqing medicine
关键词
枸杞多糖
外周血
巨噬细胞
功能
lycium bararum polysaccharides
human peripheral blood
macrophage
function
