摘要
目的:优化夏枯草中迷迭香酸的超声提取工艺,确定工艺参数的最优范围。方法:以乙醇体积分数、液固比、提取时间为考察因素,迷迭香酸提取率为评价指标,采用Box-Behnken效应面法进行试验设计并建立数学模型,采用微遗传算法对该模型进行优化,得到提取工艺参数最优范围。结果:夏枯草中迷迭香酸的最佳提取工艺为超声功率200 W,超声频率53k Hz,乙醇体积分数(50.03±1.21)%,液固比(48.28±2.27)m L·g^(-1),提取时间(47.34±1.43)min。迷迭香酸提取率0.301%。结论:Box-Behnken效应面法和微遗传算法联用时优化的迷迭香酸工艺提取率高,能得到具有浮动范围且不影响提取率、容易在生产中实现的工艺参数,可为中药提取工艺的研究提供参考。
Objective： To optimize ultrasonic extraction process ranges of rosmarinic acid from Prunellae Spica. Method： Taking ethanol concentration, liquid-solid ratio and extraction time as factors, yield of rosmarinic acid as index,mathematical model was established by Box-Behnken response surface methodology. The model was optimized by micro-genetic algorithm in order to obtain the optimum ranges of extraction process parameters. Result： Optimal conditions of extraction process were as following： ultrasonic power of 200 W,ultrasonic frequency of 53 k Hz,ethanol concentration of（ 50. 03 ± 1. 21） %,liquid-solid ratio of（ 48. 28 ± 2. 27）m L·g^-1,extraction time of（ 47. 34 ± 1. 43） min. Yield of rosmarinic acid was 0. 301%. Conclusion： This optimized process has a high yield of rosmarinic acid,it can obtain a floated wide of process parameters.
作者
曲永胜
解盈盈
郭威
周倩
王亮
QU Yong-sheng XIE Ying-ying GUO Wei ZHOU Qian WANG Liang(Shandong Academy of Chinese Medicine, Ji＇nan 250014, China College of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji＇nan 250355, China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第20期14-17,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山东省中医药科技发展计划项目(2015-167)
关键词
夏枯草
工艺参数
最优范围
微遗传算法
迷迭香酸
超声提取法
Prunellae Spica
process parameters
optimal range
micro-genetic algorithm
rosmarinic acid
ultrasonic extraction
