摘要
大豆蛋白肽具有显著的降血脂功效,但目前研究主要集中在小分子肽段,且对蛋白肽降血脂作用的分子机理探究较少.模拟豆腐蛋白的胃肠道消化,得到豆腐蛋白的体外消化物,按相对分子质量（MR）分为3 个级别：〉10 000、3 000～10 000和〈3 000,利用胆固醇胶束实验（采用两种实验基质及两种检测方法）检测其抑制膳食混合胶粒转运胆固醇的作用.结果表明,在分级的3 种豆腐消化物中,MR〉10 000具有最强的抑制膳食混合胶粒转运胆固醇作用,其胆固醇胶束吸收率为62.40%,小于阳性对照（Val-Ala-Trp-Met-Tyr,其胆固醇胶束吸收率为68.41%）,且MR〉10 000豆腐消化物的抑制胶束转运胆固醇作用随着样品质量浓度增大呈先增强后减弱的趋势.此外,胆固醇胶束实验中采用的两种“膳食混合胶粒”的转运效果及两种检测方法的结果均具有-致性,说明豆腐蛋白消化物具有影响膳食混合胶粒转运和胆固醇吸收的作用.
Soy protein peptides have significant hypocholesterolemic activity. But at present most studies are focused onsmall molecular peptides, and few explorations have been made on their hypolipidemic mechanism. In the present study, anin vitro gastrointestinal digestion model was applied to simulate the gastrointestinal digestion of tofu protein. The digestedproducts were classified into three levels： 〉 10 000, 3 000-10 000, and 〈 1 000, according to their molecular weights （MR）.The effect of these digested products on disrupting the transportation of micellar cholesterol was explored based on dietarymixed micelles （DMM） experiments with two experimental matrixes and two test methods. The results indicated that theproducts with MR 〉 10 000 had the strongest suppressing effect on transportation of micellar cholesterol. The absorption rateof micellar cholesterol in their presence was 62.40%, which was superior to the positive control, VAWWMY （Val-Ala-Trp-Met-Tyr） with an absorption rate of micellar cholesterol of 68.41%. The effect of the products with MR 〉 10 000 was enhancedfirst and then reduced with the increase in its concentration. In addition, the results of two cholesterol detection methods and thetransportation efficiencies of two cholesterol micelles were consistent. Peptides derived from in vitro digestion of tofu proteinexerted a significant inhibitory effect on the micellar solubility of cholesterol. The current work has provided the first approach toexploring the potential effect of tofu protein hydrolysates （TPH） on reducing cholesterol absorption in the intestine.
作者
董毓玭
陈责
宣佳
贾慧
赵大云
DONG Yupin CHEN Ze XUAN Jia JIA Hui ZHAO Dayun(School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, Chin)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第19期232-236,共5页
Food Science
基金
国家高技术研究发展计划(863计划)项目(2013AA102207-6)
关键词
豆腐
消化肽
膳食混合胶粒
胆固醇转运与吸收
tofu
digested peptides
dietary mixed micelles （DMM）
cholesterol transport and absorption
