摘要
为了探究猴头菇多肽作为降血脂和抗氧化食用菌功能因子的潜力,以猴头菇蛋白为原料,采用超声波-微波辅助酶法制备猴头菇多肽(Hericium erinaceus polypeptide,HEP),以多肽得率为指标,通过单因素及正交试验优化其工艺条件;采用体外抗氧化和降血脂试验对超滤分离后的不同分子质量HEP组分进行活性测定。结果表明:酶解最佳工艺参数为超声功率300 W,超声时间50 min,酶添加量5000 U/g,酶解温度50℃,多肽得率为(65.14±0.05)%;HEP-Ⅱ的DPPH·、·OH和O_(2)^(·-)清除率分别为(81.37±0.04)%,(51.17±0.03)%和(60.51±0.05)%;对牛磺胆酸钠和甘氨胆酸钠的结合率为(54.98±0.03)%和(57.74±0.01)%,对胰脂肪酶抑制率为(68.48±0.03)%,IC_(50)为(2.75±0.03)mg/mL,在pH 2.0和pH 7.0的环境下对胆固醇的结合率分别为(48.08±0.05)%和(62.89±0.07)%,表明猴头菇多肽有望成为一种天然抗氧化和辅助降血脂的功能因子。
In order to explore the potential of Hericium erinaceus polypeptide as a functional factor for hypolipidemic and antioxidation edible fungi,using Hericium erinaceus protein as raw material.the Hericium erinaceus polypeptide(HEP)was preopared by the ultrasonic-microwave assisted enzymatic method,and using polypeptide yield as an index,the process conditions were optimized by single factor and orthogonal test.The activities of HEP components with different molecular weight after ultra-filtration separation were determined by antioxidation and hypolipidemic tests in vitro.The results showed that the optimum parameters of enzymatic hydrolysis were ultrasonic power 300 W,ultrasonic time 50 min,enzyme dosage 5000 U/g,enzymatic hydrolysis temperature 50℃C,and polypeptide yield(65.14±0.05)%.The clearance rates of DPPH·,·OH and O_(2)^(·-)of HEP-Ⅱ were(81.37±0.04)%,(51.17±0.03)% and(60.51±0.05)%,respectively.The binding rate of sodium taurocholate and sodium glycocholate were(54.98±0.03)% and(57.74±0.01)%,the inhibition rate of pancrelipase was(68.48±0.03)%,and ICso was(2.75±0.03)mg/mL.The binding rate of cholesterol under the environment of pH2.0 and pH7.0 were(48.08±0.05)%and(62.89±0.07)%,respectively,indicating that HEP is expected to become a natural antioxidation and a functional factor assisting in hypolipidemic activity.
作者
同政泉
刘婷婷
张闪闪
徐新乐
王大为
TONG Zhengquan;LIU Tingting;ZHANG Shanshan;XU Xinle;WANG Dawei(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China;Scientific Research Base of Edible Mushroom Processing Technology Integration,Ministry of Agriculture and Rural Affairs,Changchun 130118,China;Engineering Research Center of Grain Deep-processing and High-efficiency Utilization of Jilin Province,Changchun 130118,China;Key Laboratory of Technological Innovations for Grain Deep-processing and High-efficiency Utiliza?tion of By-products of Jilin Province,Changchun 130118,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2024年第1期64-71,共8页
Journal of Jilin Agricultural University
基金
“十三五”国家重点研发计划重点专项(2018YFD0400204)。
关键词
猴头菇多肽
抗氧化
降血脂
超声波-微波辅助酶解
超滤分离
Hericium erinaceus polypeptide
antioxidation
hypolipidemic
ultrasonic-microwave assisted enzymolysis
ultra-filtration separation
