摘要
目的探讨高压氧预处理对大鼠脑梗死后脑水肿的影响及可能机制。方法选择SD大鼠96只,随机分为假手术组、模型组、高压氧预处理组,每组各32只。采用大脑中动脉线栓法建立大鼠大脑中动脉栓塞模型,在脑缺血后24、72 h进行神经功能评分;在脑缺血后72 h处死动物取出脑组织,检测脑梗死侧脑组织湿重干重比值(W/D),利用TTC染色法计算脑梗死体积,利用Western blot法检测脑梗死侧脑组织白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、基质金属蛋白酶9(MMP-9)蛋白的表达。结果脑梗死后24h和72 h,高压氧预处理组与模型组比较,大鼠神经功能评分明显升高(P<0.05);与模型组比较,高压氧预处理组在脑缺血后72 h脑梗死体积、脑水肿明显降低(P<0.05);与模型组比较,高压氧预处理组在脑缺血后72 h IL-1β、TNF-α、MMP-9的表达明显降低(P<0.05)。结论高压氧预处理可能通过抑制IL-1β、TNF-α、MMP-9的表达,保护血脑屏障的完整性,减轻脑梗死后脑水肿。
Objective To observe the effect of hyperbaric oxygen preconditioning to cerebral edema and possibility mechanism. Methods 96 male rats were randomly divided into sham-operated group, model group and hyperbaric oxygen preconditioning group. Focal cerebral ischemia rat models were established by occluding the middle cerebral artery（MCAO）. Neurological grades were tested 24 h,72 h after isehemia; infarct size was measured by TTC stai- ning ; the brain W/D was detected ; expressions of interleukin-1β （ IL-1β ）, tumor necrosis factior-α （ TNF-α）, ma- trix metalloproteinase 9 （MMP-9） were detected by Western blot. Results As compared with those in the modelgroup, hyperbaric oxygen preconditioning had significantly improved neurological scores at ischemia 24 h and 72 h （P 〈 0. 05 ）. After ischemia 72 h, hyperbaric oxygen preconditioning group had significantly reduced brain infarct size and brain water content as compared with the model group （ P 〈 0.05 ）. Hyperbaric oxygen preconditioning group had significantly reduced expressions of IL-1β, TNF-α, MMP-9 as compared with the model group （P 〈 0.05 ）. Conclusion Hyperbaric oxygen preconditioning significantly inhibites the expression of IL-1β, TNF-α, MMP-9 and maintains the integrity of blood brain barrier, thus, reduces the brain water content.
作者
卞合涛
初建峰
Bian Hetao Chu Jianfeng(Dept of Neurology, Jining Clinical College of Anhai Medical University, Jining First People＇ s Hospital, Jining 27200)
出处
《安徽医科大学学报》
CAS
北大核心
2016年第10期1445-1449,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81301012)
济宁市医药卫生科技项目(编号:2013jnwk26)
