摘要
目的肿瘤坏死因子-α(TNF-α)对骨髓源性肥大细胞(BMMCs)分泌MMP-3、MMP-9、IL-17的影响。方法原代培养小鼠BMMCs,将培养8周的BMMCs种植于12孔板中。细胞分为4个组:BMMCs+PBS组(对照组)、BMMCs+2 ng/m L TNF-α组(2 ng/m L组)、BMMCs+10 ng/m L TNF-α组(10 ng/m L组)、BMMCs+50 ng/m L TNF-α组(50 ng/m L组),不同浓度TNF-α与BMMCs作用后,在12、24 h收集细胞,用实时荧光定量逆转录聚合酶链式反应(real-time polymerase chain reaction,real-time PCR),在m RNA水平检测MMP-3、MMP-9、IL-17的表达。结果培养12 h后,2 ng/m L组、10 ng/m L组、50 ng/m L组MMP-3、MMP-9、IL-17 m RNA的表达均高于对照组(P<0.05),并且随着TNF-α浓度的上升m RNA的表达显著升高(P<0.05)。24 h结果与12 h结果一致,24 h与12 h之间比较,除50 ng/m L组MMP-3 m RNA的表达无差异(P>0.05),其余各组之间MMP-3、MMP-9、IL-17m RNA的表达显著升高(P<0.05)。结论 TNF-α可以上调BMMCS表达MMP-3、MMP-9、IL-17,且具有浓度依赖性和时间依赖性。
Objective To investigate the effect of tumor necrosis factor-α（TNF-α） on the release of matrix metalloproteinase-3（MMP-3）, MMP-9, and interleukin-17（IL-17） in cultured mouse bone marrow-derived mast cells（BMMCs） in vitro. Methods Primarily cultured mouse BMMCs at 8 weeks were exposed PBS（control） or TNF-α at the concentrations of 2, 10, or 50 ng/m L for 12 or 24 h. Real-time PCR was performed to detect the m RNA expressions of MMP-3, MMP-9, and IL-17 in the exposed cells. Results A 12-hour exposure of the BMMCs to TNF-α caused significantly increased expressions of MMP-3, MMP-9, and IL-17 in a concentration-dependent manner（P〈0.05）. Prolonged exposures of the cells to 2 and 10 TNF-α for 24 h further increased MMP-3, MMP-9, and IL-17 m RNA expressions, but exposure to 50 ng/m L TNF-α for 24 h increased only MMP-3 and MMP-9 expressions but not IL-17 m RNA expression. Conclusions TNF-α treatment of primarily cultured BMMCs can significantly increase the cellular expressions of MMP-3, MMP-9, and IL-17 m RNA in a time- and dose-dependent manner.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2015年第11期1633-1637,共5页
Journal of Southern Medical University
基金
国家自然科学基金(81172875)~~
关键词
骨髓源性肥大细胞
肿瘤坏死因子-Α
MMP-3
MMP-9
IL-17
bone marrow-derived mast cells
tumor necrosis factor-α
matrix metalloproteinase-3
matrix metalloproteinase-9
interleukin-17
