摘要
目的:探讨耐力运动对糖尿病小鼠骨骼肌AMPK/SIRT1/NF-κB炎症信号通路及相关炎症因子的影响,以其从骨骼肌慢性炎症的角度为肥胖、糖尿病等代谢类疾病的防治提供新的研究靶向。方法:采用4周高脂膳食喂养加注射链脲佐菌素(Streptozotocin,STZ)的方法构建糖尿病小鼠模型,建模成功后随机分为安静对照组(C)、运动对照组(E)、糖尿病安静组(D)、糖尿病运动组(DE)。E组和DE组进行6周游泳耐力运动,1h/天,5天/周。末次运动结束,空腹12h后处死小鼠并采样。RT-PCR及Western Blotting技术检测相关基因的mRNA及蛋白表达水平。结果:1)与C组相比,D组小鼠体重极显著降低(P<0.01),空腹血糖显著升高(P<0.01);与C组相比,E组小鼠体重显著降低(P<0.05);与D组相比,DE组小鼠空腹血糖显著降低(P<0.05);与E组相比,DE组小鼠体重极显著性降低(P<0.01),空腹血糖极显著性升高(P<0.01)。2)与C组相比,D组小鼠IL-10mRNA表达极显著性降低(P<0.01),E组IL-6 mRNA表达显著性升高(P<0.05),IL-10 mRNA表达极显著性升高(P<0.01);与D组相比,DE组TNF-αmRNA表达显著降低(P<0.05),IL-10 mRNA表达极显著性升高(P<0.01);与E组相比,DE组IL-6 mRNA表达显著性下降(P<0.05)。3)与C组相比,D组AMPK蛋白表达和AMPK活性显著性下降(P<0.05),NF-κBp65 mRNA表达显著性升高(P<0.05);与D组相比,DE组AMPKα2及SIRT1 mRNA表达极显著性升高(P<0.01),AMPK及p-AMPK蛋白表达和AMPK活性显著性升高(P<0.05),NF-κBp65蛋白表达极显著性下降(P<0.01);与E组相比,DE组SIRT1 mRNA表达极显著性升高(P<0.01)。结论:长期耐力运动可抑制糖尿病小鼠骨骼肌中促炎因子的基因表达,促进抗炎因子的基因表达,同时,AMPK/SIRT1/NF-κB炎症信号通路的被抑制效应得到缓解。
Objective：The purpose of the present study was to investigate the effect of endurance exercise on inflammatory factors and corresponding signaling pathways AMPK/SIRT1/NF-κB in the skeletal muscles of diabetic mice,hoping to provide new evidence for the prevention and treatment of obesity and diabetes from the perspective of inflammation of the skeletal muscle.Methods：The diabetic mice were established by feeding with high-fat diet for 4 weeks,and then injected with Streptozotocin.All the mice were randomly chosen and assigned to either control group（C,n=8）or exercise group（E,n=8）,diabetic group（D,n=8）or diabetic exercise group（DE,n=8）,Groups E and DE were then interfered with 6-week swimming exercise of moderate intensity,one hour per day,five days per week.RT-PCR was used to measure the mRNA expression of TNF-α,CCL2,IL-6,IL-10,AMPKα1,AMPKα2,SIRT1 and NF-κBp65.Western Blotting was used to measure the protein level of AMPK,p-AMPK,SIRT1,NF-кBp65 and AC-NF-кBp65.Results：1）Compared with group C,the weight of mice in group D decreased significantly（P〈0.01）,meanwhile the fasting blood glucose increased significantly（P〈0.01）;Compared with group C,the weight of mice in group E decreased significantly（P〈0.05）;Compared with group D,the fasting blood glucose in group DE decreased significantly（P〈0.05）;Compared with group E,the weight of mice in group DE decreased significantly,while the fasting blood glucose increased significantly（P〈0.01）.2）Compared with group C,the IL-10 mRNA expression in group D was decreased significantly（P〈0.01）;while,the IL-6 mRNA expression in group E was increased significantly（P〈0.05）;Compared with group D,the TNF-αmRNA in group DE was decreased significantly（P〈0.05）,the IL-10 mRNA in group DE was increased significantly（P〈0.01）;Compared with group E,the IL-6 mRNA of group DE was decreased significantly（P〈0.05）.3）Compared with group C,the protein expression and activity of AMPK were decreased significan
出处
《体育科学》
CSSCI
北大核心
2016年第9期40-47,共8页
China Sport Science
基金
国家自然科学基金资助项目(31671241)
