摘要
目的研究不同运动对二甲双胍干预的db/db小鼠血糖、血脂代谢、骨骼肌炎性因子的影响。方法将24只6~8周龄db/db雄性小鼠随机分为对照组(C,n=8)、抗阻组(P,n=8)和有氧组(O,n=8)。经过8周运动干预后,每周监测小鼠血糖。酶联免疫吸附测定(ELISA)法检测小鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量,Western Blot法检测骨骼肌中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量。结果训练后,P组、O组血糖均降低,O组更为显著,差异有统计学意义(P<0.05);训练后,P组、O组TC、TG、LDL-C、HDL-C水平优于C组,差异有统计学意义(P<0.05);训练后,P组、O组骨骼肌中IL-6水平高于C组,差异有统计学意义(P<0.05)。结论有氧运动结合二甲双胍对降低血糖、改善血脂代谢及缓解骨骼肌炎症的疗效均优于抗阻结合二甲双胍。
Objective To study the effects of different exercises on blood glucose,blood lipid metabolism and inflammatory factors of skeletal muscle in db/db mice interfered with metformin.Methods Twenty-four db/db male mice aged 6-8 weeks were randomly divided into control group(C,n=8),resistance group(P,n=8)and aerobic group(O,n=8).After 8 weeks of exercise intervention,the mice's blood glucose was monitored weekly.ELISA method was used to detect the content of TC,TG,LDL-C,HDL-C in the serum of mice,and the content of TNF-αand IL-6 in skeletal muscle was detected by Western Blot method.Results After training,the blood sugar of group P and O decreased,and group O was more significant,and the difference was statistically significant(P<0.05);after training,group P and O had excellent levels of TC,TG,LDL-C,and HDL-C in group C,the difference was statistically significant(P<0.05);after training,the levels of IL-6 in the skeletal muscle of group P and O were higher than those of group C,and the difference was statistically significant(P<0.05).Conclusion The effects of aerobic exercise combined with metformin on lowering blood sugar,improving blood lipid metabolism and alleviating skeletal muscle inflammation are better than those of resistance combined with metformin.
作者
俞莹莹
赵彦
谭朝文
郑玉婵
YU Yingying;ZHAO Yan;TAN Zhaowen;ZHENG Yuchan(School of Sports and Health,Nanjing Institute of Physical Education,Nanjing,Jiangsu Province,210000 China)
出处
《糖尿病新世界》
2021年第11期15-18,22,共5页
Diabetes New World Magazine
基金
2018年“江苏省研究生教育创新工程”项目(KYCX18-1728)。
