摘要
目的:探讨不同浓度二甲双胍对SD大鼠脂肪代谢相关基因Galectin-3、PPARγmRNA及蛋白表达的影响。方法:选取医院动物中心的成年SD雄性大鼠30只,体质量175~195 g,按照随机数字表法分为A、B及C组,每组10只,分别腹腔注射二甲双胍0、45及180 mg/kg处理72 h后,无菌状态下取出皮下和肾脏周围脂肪,检测脂肪代谢相关基因Galectin-3、PPARγ的mRNA及蛋白的表达和炎性因子C反应蛋白(CRP)、白细胞介素1β(IL-1β)的mRNA表达并进行统计学分析。结果:C组大鼠Galectin-3 mRNA相对表达明显低于A、B组,且B组大鼠明显低于A组;C组大鼠PPARγmRNA相对表达明显高于A、B组,上述差异均有统计学意义(P<0.05)。C组大鼠CRP、IL-1βmRNA相对表达明显低于A、B组,且B组大鼠明显低于A组,差异均有统计学意义(P<0.05)。C组大鼠Galectin-3蛋白相对表达明显低于A、B组,且B组大鼠明显低于A组;C组大鼠PPARγ蛋白相对表达明显高于A、B组,且B组明显高于A组,上述差异均有统计学意义(P<0.05)。结论:二甲双胍可抑制脂肪代谢基因Galectin-3的mRNA及蛋白的表达,促进成脂基因PPARγ的mRNA及蛋白的表达,增加CRP、IL-1β的mRNA表达水平,呈一定的浓度依赖关系,可从多方面抑制脂肪合成,对肥胖和糖代谢障碍疾病患者有重要意义。
OBJECTIVE:To probe into the effects of metformin at different concentrations on the mRNA and protein expression of fat metabolism-related genes of Galectin-3 and PPARγin rats. METHODS:30 adult SD male rats in hospital animal center were selected, weighed 175-195 g, which were divided into groups A, group B and group C via random number table, with 10 rats in each group. After the three groups were given metformin injection at 0, 45 and 180 mg/kg respectively for 72 h, mRNA and protein expression of fat metabolism-related genes of Galectin-3 and PPARγ and mRNA expression of inflammatory factor C-reactive protein(CRP) and interleukin-1β(IL-1β) were detected by extracting subcutaneous and peri-renal fat under aseptic conditions, so as to conduct statistical analysis. RESULTS: The relative mRNA expression of Galectin-3 in group C was significantly lower than that of group A and group B, and that of group B was significantly lower than group A;the relative mRNA expression of PPARγ in group C was significantly higher than that of group A and group B, with statistically significant differences(P<0.05). The relative mRNA expressions of CRP and IL-1β in group C were significantly lower than those of group A and group B, and those of group B were significantly lower than group A, with statistically significant differences(P<0.05). The relative protein expression of Galectin-3 in group C was significantly lower than that of group A and group B, and that of group B was significantly lower than group A;the relative protein expression of PPARγ in group C was significantly higher than that of group A and group B, and that of group B was significantly higher than group A, with statistically significant differences(P<0.05). CONCLUSIONS: Metformin can inhibit the mRNA and protein expression of fat metabolism-related genes of Galectin-3, promote the mRNA and protein expression of adipogenesis gene of PPARγ, improve the mRNA expression levels of CRP and IL-1β, showing a certain concentration dependence,which can inhibit fat
作者
兰天
刘巍梦
邹阳
邱平
LAN Tian;LIU Weimeng;ZOU Yang;QIU Ping(Dept.of Endocrinology,the First Affiliated Hospital of Chengdu Medical College,Sichuan Chengdu 610500,China;Dept.of Gynecology,the Sixth People’s Hospital of Panzhihua,Sichuan Panzhihua 617023,China;Dept.of Internal Medicine,the Ninth People’s Hospital of Chongqing,Chongqing 400700,China)
出处
《中国医院用药评价与分析》
2019年第11期1299-1302,共4页
Evaluation and Analysis of Drug-use in Hospitals of China
基金
四川省教育厅科研项目(No.18ZB0167)
