摘要
目的建立HPLC法同时测定博尔宁胶囊（重楼、女贞子、大黄等）中5种成分的含有量。方法博尔宁胶囊乙醇提取液的分析采用Hypersil C18色谱柱（250 mm×4.6 mm,5μm）;以乙腈-甲醇（1∶1）-水为流动相,梯度洗脱;体积流量1.3 m L/min;柱温30℃;检测波长203 nm（重楼皂苷Ⅶ、重楼皂苷Ⅵ和重楼皂苷Ⅰ）和224 nm（女贞苷和特女贞苷）;进样量20μL。结果重楼皂苷Ⅶ在6.70～134.00μg/m L（r=0.999 8）、重楼皂苷Ⅵ在4.35～87.00μg/m L（r=0.999 6）、重楼皂苷Ⅰ在6.48～129.60μg/m L（r=0.999 9）、女贞苷在7.45～149.00μg/m L（r=0.999 7）、特女贞苷在7.03～140.60μg/m L（r=0.999 6）范围内线性关系良好,平均回收率（n=6）分别为99.28%、96.72%、99.30%、96.93%和98.68%,RSD分别为1.18%、1.05%、0.60%、1.42%和0.79%。结论该方法准确灵敏,重复性好,可用于博尔宁胶囊的质量控制。
AIM To develop an HPLC method for the simultaneous determination of contents of five constituents in a supplementary antitumor agent,Bo＇erning Capsules（ Paridis Rhizoma,Ligustri Lucidi Fructus,Rhei Radix et Rhizoma,etc.）. METHODS The analysis of Bo＇erning Capsules ethanolic extract was performed on a30 ℃ thermostatic Hypersil C18column（ 250 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of acetonitrile-methanol（ 1 ∶ 1）-water flowed at 1. 3 m L / min in a gradient elution manner. The detection wavelengths were set at 203 nm for polyphyllin Ⅶ,polyphyllin Ⅵ and polyphyllin Ⅰ,and 224 nm for ligustroflavone and specnuezhenide. And the injection volume was 20 μL. RESULTS PolyphyllinⅦ,polyphyllin Ⅵ,polyphyllinⅠ,ligustroflavone and specnuezhenide showed good linear relationships within the ranges of 6. 70- 134. 00 μg / m L（ r =0. 999 8）,4. 35- 87. 00 μg / m L（ r = 0. 999 6）,6. 48- 129. 60 μg / m L（ r = 0. 999 9）,7. 45- 149. 00 μg / m L（ r = 0. 999 7） and 7. 03- 140. 60 μg /m L（ r = 0. 999 6）,whose average recoveries（ RSD） were 99. 28%（ 1. 18%）,96. 72%（ 1. 05%）,99. 30%（ 0. 60%）,96. 93%（ 1. 42%） and 98. 68%（ 0. 79%）,respectively. CONCLUSION This accurate,sensitive and well reproducible method can be used for the quality control of Bo＇erning Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2016年第9期1952-1955,共4页
Chinese Traditional Patent Medicine
基金
安徽省高等学校教学质量与教学改革工程实训中心建设项目(20101460)
