摘要
[目的]建立贞芪扶正颗粒中特女贞苷含量测定方法。[方法]采用Thermo Syncronis C18(4.6×250mm,5μm)、Agilent ZORBAX SB-AQ(4.6×250mm,5μm)、SPOLAR C18(4.6×250mm,5μm);十八烷基硅烷键合硅胶为填充剂-C18(4.6×250mm,5μm);柱温:30℃;检测波长:224nm;流动相:甲醇-水(40:60);流速:1.0m L/min-1。[结果]精密度RSD=0.4%,重复性RSD=1.0%,稳定性RSD=0.9%,耐用性理论板数均>3000,加样回收率RSD=0.78%。限定贞芪扶正颗粒中特女贞苷含量不得低于0.21mg/g。[结论]高效液相色谱法测定贞芪扶正颗粒特女贞苷含量,结果准确、可靠,可用于贞芪扶正颗粒质量控制。
[Objective] To establish the Method for determining the content of Specnuezhenide in Zhenqi Fuzheng granule.[Method] Thermo Syncronis C18（4.6 × 250mm, 5 μm）,Agilent ZORBAX SB- AQ（4.6 × 250mm, 5 μm）,SPOLAR C 18（4.6 × 250mm, 5 μm）; octadecyl silane for the bonded silica filler-C18 （4.6 × 250mm, 5 μm）; column temperature： 30℃; dtermine wave length： 224nm; mobile phase： methyl alcohol- water （40：60）; flow rate： 1.0mL/min-l.[Results] accuracy： RSD=0.4%, repeatability： RSD=1.0%, stability： RSD=0.9%, every durability theoretical plates 〉 3000, recovery rate： RSD=0.78%. Limination of Specnuezhenide should not be less than 0.21mg/g. [Conclusion] the result is accuracy, Method of HPLC is reliable, can be used to control the quality of Zhenqi Fuzheng granule.
出处
《实用中医内科杂志》
2015年第3期135-136,共2页
Journal of Practical Traditional Chinese Internal Medicine
基金
基金资助:国家评价性抽验
