摘要
目的：建立女贞子中红景天苷含量测定的RP-HPLC法。方法：样品溶液经大孔树脂和聚酰胺柱层析纯化，采用Hypersil C18色谱柱（4．6mm×150mm，5μn），流动相为甲醇：水（20：80），流速0．4mL／min。检测波长220nm。结果：红景天苷在1～80gg／mL范围内线性关系良好（r=0．9999），高、中、低浓度（40、10、5μg／mL）加样回收率分别为（101．4±1．52）％、（95．6±1．45）％、（100．5±2．18）％（n=5）。浓度为80、40、10μg／mL的红景天苷日内RSD分别为0．28％、0．17％、0．48％（n=5）；日间RSD分别为0．56％、0．06％、0．12％（n=5）。稳定性的RSD=1．64％，表明红景天苷在24h内稳定。应用该法测定女贞子中红景天苷的含量为0．718mg／g。结论：本法简便、快捷、准确，适用于女贞子中红景天苷的含量测定。
Objective： To establish a RP HPLC method for determining the content of salidroside in fruits of Ligustrum lueidum Ait.. Methods： The sample was purified by macroreticular resin and polyamide column. The RP HPLC separation was accomplished on Hypersil C18 column （4.6 mm×150 mm,5μm） with mobile phase of methanol ： water （20：80） and flow rate of 0.4 mL/min. The detection wavelength was 220 nm. Results： The linear correlation of salidroside was observed within the range of 1-80μg/mL （r=0. 999 9）. The recovery rates of high, middle and low concentrations （40, 10, 5μg/mL） in samples were （101. 4±1. 52）% , （95.6±1.45）% and （100.5±2.18）% （n=5）, respectively. The intra-day relative stand- ard deviation for concentrations of 80, 40 and 10μg/mL, were 0. 28% ,0. 17% and 0. 48% （n=5）, respectively; and inter-day RSD were 0.56% ,0.06% and 0.12% （n=5 ）, respectively. The stability RSD was 1.64%, so salidroside was stable in 24 hours. The content of salidroside extracted was 0. 718 mg/g by the determination. Conclusion： This method is proved to be simple, rapid, accurate and suitable for the quality control of salidroside in Fructus Ligustrum Lucidum Air.
出处
《药学服务与研究》
CAS
CSCD
2006年第2期118-120,共3页
Pharmaceutical Care and Research
基金
上海市科委中药现代化重大专项科研计划项目(No.03DZ19526)
关键词
女贞子
红景天苷
色谱法
高压液相
Fructus Ligustrum lucidum Air.
salidroside
chromatography, high pressure liquid
