摘要
目的通过星点设计-效应面法优化茵陈蒿汤(茵陈、栀子、大黄)配方颗粒的提取工艺。方法在单因素试验基础上,以绿原酸、栀子苷、6,7-二甲氧基香豆素、芦荟大黄素、大黄酸、大黄素、大黄酚含有量和干膏收率为指标,以茵陈先煎时间、后下煎煮时间、加水量为因素,星点设计-效应面法对提取工艺进行优化。结果最佳条件为茵陈先煎时间46 min,后下煎煮时间23 min,加水量730 m L,总评"归一值"0.794,与预测值(0.813)相当。结论该方法简便可靠,重复性好,可用于优化茵陈蒿汤配方颗粒的提取工艺。
AIM To optimize the extraction technique for Granulated Yinchenhao Decoction（ Artemisiae Scopariae Herba,Gardeniae Jasminoidis Fructus and Rhei Radix et Rhizoma） by central composite design-response surface method. METHODS On the basis of single factor test,with the contents of chlorogenic acid,geniposide,6,7-dimethoxy-2-benzopyrone,aloe-emodin,rhein,emodin,chrysophanol,and dry extract yield as indices,the extraction was optimized through control of different administration timing（ decocting of Artemisiae Scopariae Herba prior to or later than other drugs） and water consumption of Artemisiae Scopariae Herba by central composite designresponse surface method. RESULTS The best conditions were determined to be 46 min pre-decocting time and23 min delayed casting of Artemisiae Scopariae Herba,and 730 m L of water consumption. The 0. 794 overall desirability value approximated the predictive value（ 0. 813）. CONCLUSION This simple,reliable and well reproducible method can be used to optimize the extraction technique for Granulated Yinchenhao Decoction.
出处
《中成药》
CAS
CSCD
北大核心
2016年第9期1938-1942,共5页
Chinese Traditional Patent Medicine
基金
国家"十一五"科技支撑计划(2009BAI84B04)
关键词
茵陈蒿汤颗粒
提取工艺
星点设计-效应面法
Granulated Yinchenhao Decoction
extraction
central composite design-response surface method
