摘要
目的:确定茵陈蒿汤水提液的高效液相色谱分析方法。方法:色谱柱为LichrospherC18柱(4.6mm×25cm,5臁m),采用A相(0.5磷酸水溶液)、B相(0.5‰磷酸甲醇溶液)为流动相进行非线性梯度洗脱(A相起始比例为90%,50min内下降至40%,其后的5min内下降至28.5%);流速1ml/min,检测波长为254nm,柱温:25℃,进样量:5臁L=果:运用该梯度洗脱方法能较好的分离茵陈蒿汤水提液中的各个成分。结论:本方法较完整的呈现了茵陈蒿汤水提液的化学成分组成,能为茵陈蒿汤的指纹图谱研究提供参考。
Objective： To establish an optimal method to analyze ＂Yinchenhao decoction＂ by RP-HPLC. Methods： Lichrospher C,, column （4.6 mm×25 cm, 5 pro） with temperature at 25 ℃ was used with a mobile phase of A （0.5 ‰ phosphoric acid ） ,B（methanol with 0.5 ‰ phosphoric acid）, used a non-linear gradient of 90 % to 40 %.A phase in 50 minutes and from 40 % to 28.5 % in the followed 5 minutes mixed with B phase. The elution was performed at the flow rate of 1 ml/ rain. UV detection wavelength was 254 nm, the injection volume was 5 μL. Results： The ingredients in Yinchenhao decoction were well seperated by gredient elution. Conclusion： The ingredients in Yinchenhao decoction were well presented in the chromatographic detection via this method, and the method can be applied for the study of the fingerprints of Yinchenhao decoction.
出处
《抗感染药学》
2006年第2期54-55,88,共3页
Anti-infection Pharmacy
