摘要
目的:建立高速逆流色谱(HSCCC)法分离制备山芝麻中三萜类成分的方法。方法:山芝麻乙酸乙酯萃取部位采用二步分离法进行HSCCC分离;所得各组分用质谱与核磁共振波谱法进行结构鉴定,并用红外光谱法进行表征分析。结果:从山芝麻乙酸乙酯萃取部位分离制备得到6个化合物,各化合物经鉴定为:山芝麻宁酸甲酯(1)、山芝麻酸甲酯(2)、山芝麻宁酸(3)、山芝麻酸(4)、白桦脂酸(5)、齐墩果酸(6)。6个化合物进行HPLC分析,质量分数均大于95.0%。结论:HSCCC适用于分离制备山芝麻中的三萜类成分,方法快速,可以实现产品高纯度和高得率的制备。
Objective： To develop a method for separation and purification of triterpenoids from Helicteres angustifolia by highspeed countercurrent chromatography（ HSCCC）. Methods： The ethyl acetate extract of Helicteres angustifolia were separated by two-step HSCCC. The structures of the target compounds were identified by ESI-MS and1H-NMR and characterized by FTIR. Results： Six compounds were isolated and prepared from ethyl acetate extract of Helicteres angustifolia,and identified as methyl helicterilate（ 1）,methyl helicterate（ 2）,helicterilic acid（ 3）,helicteric acid（ 4）,betulic acid（ 5）,oleanolic acid（ 6）. The purity of the chemical constituents were determined by HPLC,and the mass fraction was more than 95. 0%,respectively. Conclusion： This method is suitable for the separation and preparation of triterpenoids from Helicteres angustifolia,which is rapid,high purity and high yield.
出处
《中药材》
CAS
CSCD
北大核心
2016年第5期1053-1056,共4页
Journal of Chinese Medicinal Materials
基金
广东省中山市科技计划项目(2014A2FC245)
广东省中山市科技计划项目(20132A162)
关键词
高速逆流色谱
山芝麻
三萜
High-speed countercurrent chromatography（HSCCC）
Helicteres angustifolia L.
Triterpenoids
