摘要
采用SRAP分子标记技术对重庆地区的16份锦橙(Citrus sinensis Osbeck)资源进行遗传鉴定,结果显示:锦橙资源间的亲缘关系非常接近。在16份锦橙的PCR扩增中,80对SRAP引物中只有30对引物扩增出了差异带,多态性引物比例为37.5%,共扩增出56份特征带,平均每对引物扩增出1.87条差异带。聚类结果显示,八楞锦橙和长叶橙与其他锦橙类型遗传距离较远,而遗传关系最近的是BDQ222与BDM221、S26。SRAP的鉴定结果显示,此标记在鉴定锦橙这种亲缘关系非常近的芽变类型上具有较高的可应用性。进一步对部分差异片段测序分析,推测这些差异片段有可能与锦橙的变异性状相关。
80 pairs of SRAP primer were used to analyze the genetic relationship among 16 Jincheng （Citrus sinensis Osbeck） germplasms from Chongqing area.The results showed that the genetic relation- ship between different Jincheng species was very close.In the PCR amplification,only 30 pairs of primers （37.5 %） were polymorphic.56 polymorphic bands were obtained, with an average of 1.87 band each pair of primers.Results of cluster analysis showed that the genetic relationship between Baleng Cheng and Changye Cheng was more far than that between other types. The closest relationship was between BDQ222 and BDM221,and between BDQ222 and S26.SRAP markers have a high applicability in identif- ying plant types with close genetic relationship.By sequencing and analyzing polymorphic fragments,we speculate that some polymorphic fragments may be associated with variation of some traits of Jincheng. It will provide basis for future exploring Jineheng germplasm.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2016年第3期1-5,共5页
Journal of Huazhong Agricultural University
基金
重庆市基本科研业务费专项(2013cstc-jbkg-00534)
国家现代柑橘产业技术体系(SARS-27)
关键词
锦橙
品种资源
芽变
SRAP
遗传鉴定
Jincheng（Citrus sinensis Osbeck）
germplasm
bud variation
SRAP
genetic identifi-cation
