摘要
为改良罗氏沼虾(Macrobrachium rosenbergii)的养殖特性,将缅甸引进种群(BP)和浙江本地种群(ZP)杂交,获得了4个杂交群体,A、B、C、D分别为BP(♀)与ZP(♂)、ZP(♀)与BP(♂)、ZP(♀)与B(♂)、ZP(♀)与A(♂)的杂交子一代。比较BP、ZP、A、B、C、D 6个不同群体的繁殖和生长性能。结果表明,BP、B、C、D的抱卵率显著高于ZP和A群体,BP、A、B、D平均出苗量显著高于ZP和C群体。在快速生长期(8月2日至9月28日)BP群体的生长速度显著高于ZP、B、C群体,A群体显著高于ZP、B群体,D群体显著高于ZP群体;其他时期各群体间差异不显著。应用SRAP(Sequence-related amplified polymorphism)对BP、ZP、A、B、C、D 6个不同群体的遗传多样性进行比较分析,其多态位点比例分别是60.3%、55.6%、60.7%、63.3%、61.9%、64.1%,遗传多样指数(H0)分别为0.185、0.175、0.196、0.192、0.193、0.197。以上结果表明,罗氏沼虾缅甸引进种的遗传多样性高于浙江本地种,同时通过杂交可提高遗传多样性,改良养殖性状。[中国水产科学,2006,13(4):667-673]
Giant freshwater prawn （Macrobrachiurn rosenbergii ） belongs to Macrobrachium, Decapoda, Palaemonidae. It is one of the most popular freshwater prawns cultivated in China after introduced from Malaysia last century. However, their producing traits were degenerated apparently because of inbreeding for twenties years. So we imported some prawns from Burma to mate with the Zhejiang cultured populations to improve their culture characters. Four populations, designated A, B, C and D, were obtained respectively from crossings between Burma introduced population （BP）（ ♀ ）and Zhejiang cultured population （ZP）（♂）, ZP（♀） and BP（♂）, ZP（♀） and B（♂）, ZP （♀） and A（♂）. In the study, six populations （BP, ZP, A, B, C and D） were compared on the ability of reproduction and the growth velocity. The results demonstrated the egg-carrying capability of BP, B, C and D was significantly higher than ZP and BP, the average zoea output of BP, A, B and D was significantly higher than that of ZP and C. During the rapid growth period （from Aug 2 to Sep 28）, the growth velocity of BP was significantly higher than that of ZP, B and C, A growed significantly faster than ZP and B, D significantly faster than ZP, and no significant difference between the other populations was found. It is the first report of application of SRAP （sequence-related amplified polymorphism） to make a comparative analysis on genetic diversity of giant freshwater prawn among six groups （BP, ZP, A, B, C and D）. The proportion of polymorphic loci was 60.3%, 55.6%, 60.7%, 63.3%, 61.9% and 64.1% in six populations and Shannon index Ho was 0. 185, 0. 175, 0. 196, 0.192, 0. 193 and 0. 197, respectively. It suggests that the genetic diversity of introduced population is higher than that of cultured population. All the results above mentioned demonstrated that the genetic diversity and cultural traits could be improved by crossing with the introduced population. And the SRAP markers identified in this st
出处
《中国水产科学》
CAS
CSCD
北大核心
2006年第4期667-673,共7页
Journal of Fishery Sciences of China
基金
浙江省重大科技攻关项目(2003C12022)
关键词
罗氏沼虾
繁殖性能
生长速度
杂交优势
SRAP
遗传多样性
Macrobrachium rosenbergii
reproductive ability
growth velocity
genetic diversity
SRAP
genetic diversity
