摘要
对一大白菜隐性细胞核雄性不育系454AB的恢复基因BrMsf3进行了SRAP标记,构建包含320个单株的分离群体,筛选SRAP标记1128个,筛选出与恢复基因BrMsf3连锁的2个标记BMe10SA4和M52K2,与恢复基因BrMsf3的遗传距离为4.35cM和7.74cM。
Genic male sterility lines were widely used in F1 seed production in Chinese cabbage(Brassica rapa L.ssp.pekinensis).A recessive genic male sterile line 454AB which contained 320 individuals was constructed for this study.SRAP techniques and bulked segregant analysis(BSA)were used to screen markers linked to the RGMS restoring gene.Among the 1 128 primer combinations,only BMe10SA4 and M52K2 showed polymorphism between bulks of male sterile and fertile.The genic distances between restoring gene with BMe10SA4...
出处
《中国蔬菜》
北大核心
2011年第4期13-16,共4页
China Vegetables
基金
国家自然科学基金(30571273)
国家"863"计划(2006AA100108
2008AA10Z154)
"十一五"国家科技支撑计划(2008BADB1B01-1)
农业部园艺作物遗传改良重点开放实验室项目
关键词
大白菜
细胞核雄性不育
分子标记
Chinese cabbage
Genic male sterility
Molecular marker
